| Infertility is one of the comfnon Gynecology diseases. Most Datientscan obtain an exPlanation for infertility through the systematicinfertility evaluation. But it was estimated that l5% to20%of couplesundergoing evaluation for inferti l ity eventually have no cause identi fiedfor their failure to conceive. In the IVF--ET, the implantation rate wasalways low in contrast to the relatively high ovum fertil ization. Thesephenomenons cause us to attend more attention to the defects inimplantation in woman with infertil i ty. Successful implantation is acomDlex and exquisite process, which requires the synchronization ofembryo development and endometrial preDaration. lt was also regulated bylots of cytokines, growth factors, adhesive molecular, et al.Unexplained infertility refers to the failure of a couple to conceivein whom no definable cause for infertility can be identified. Recentstudies in the factor that participate and modulate the process ofperi--â…¡nP1antation have revea1ed that the diminished receptivity causedby the disturbance of the endometria1 1oca1 modu1ator factors, resu1tingin fai 1 ed or defective embryo imp1antation i s a mechani sm that may accountfor the unexp1ained lnferti1 ity.The 1oca11y produced IGFs are be1ieved to mediate the effects ofestrogen and progesterone on endomtria1 growth and differentiation. T1JeIGFs have the pro1iferation, differentiation and antiapoptoticpropeTties. 1GF-- I mRNA are high1y abundant during the pro1iferate phaseof the cyc1e, but re1ative1y Teduced in the secretary phase. 1GF--â…¡ geneexpression, in contrast, demonstrated an opposite trend, being 1ow inthe pro1iferate phase, high in the secretary phase and in ear1y Dregnancydecidua. The near1y exc1usive expression of IGF--1I comDared to 1GF-- IchAs in the secretary phase and in ear1y Dregnancy decidua suggest thatIGF-- I1 may play a ro1e in the progress of decidulization and imP1antation.The re1ationship between the endometria1 IGF-- â…¡ and unexp1ainedinferti1ity haven't been reported so far.1n this study, 18 patients with the diagnosis of unexp1ainedinferti 1ity were des1gnated to the study grouD' The median duration oftheir inferti 1 ity was 3. 331 2. 72 years (range 2--13year). 0f these Datient14 had primary inferti1ity and four had secondary inferti1ity. 15 womenwith norma1 ferti1ity were se1ected as feTti1ity contro1s. Every womanrneasured BBT dai 1y, ovu1ation was considered to have occurred when therewas a temDerature rise of 30. 05'C, for312 days immediate Dreceding amenstruation period. Six to nine days afteT the onset of BBT ascending,venous b1ood sanp1es were co11ected and the endometria1 biopsies wereperformed. The b1ood serun and the tissue were both stored at the is0C. Serum E, and p were measured by specific RIA. The SPimmunohistochemica1 techniques with the monoc1onoa1 antibody for theIGF--â…¡ was performed on crystated sections of endometria1 biopsies. Forthe PR was performed on paraffin wax sections. Routine HE staining wasaccomp1ished in a11 women. Immunostaining outcome was semi--quantifiedby the HSC0RE. The purDose of this study was to investlgateimmunohistochemica1 1oca1ization of 1GF-- â…¡ in mid--secretary phaseendometrium and to compare staining intensity between in inferti 1 ity andthe parous contTo1s, to discuss the significance in the disturbance ofthe 1oca11y endometria1 factor on the pathogenic mechani sm of inferti 1 ityand further to confirm the action of 1GF-- â…¡ in modu1ating the endometrâ…¡nrecept ivi ty.Resu 1 t s1. IGF--ll were present in cytop1asm. The immunostaining intensity ing1andu1ar was a 1itt1e stronger than that of stroma1. The g1andu1arimfnunostaining score was 1ower in the study group, compared that with thecontro1s (159. 00 I 42. 35 versus 125. 27 1 41. 91, D(0. 05). The storma1immunostaining score didn't differ between the two groups (114. 5f28. 7versus 107' 50t27. 83, P>0. 05)2. PR staining w... |
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