| Purpose: This paper is intended to probe for the possiblemechanism of sensorineural hearing loss caused by iron deficiencythrough the observation of genesis of sensorineural hearing loss in rats atdefferent periods of time of iron deficiency, the vdriation of relativecontent of actin in cochlear tissue and the relation between theimmunohistoclunical results of actin in cochlear hair cells and thevariation of auditory threshold.Matnods:l06 healthy Wistar rats between 20-25 days of age(26-45g) were randomly divided into control and iron deficiency groups.The experimental models were prepared by iron-deficient diet. Theanimals at the 4～(th), 8～(th) and 12～(th) week were determined by ABR,SDS-PAGE, Westem blot and immnohistochemical methods. Therelationship between the variation of content and distribution of cochlearactin at different iron-deficient period and the changes of ABR thresholdwas observed.Results: The incidence of sensorineural hearing loss at week 4, 8and l2 in ID group was 26.7%, 37.0% and 42.9% respectively Thedifference between the relative content of actin and immunohistochemical5findings in iron-deficicient rats with normal hearing at experimentalperiod and those in controls were not significan (P>0.05) while the actincontent of drops obviously in iron-deficient rats with hearing loss, and theimmuno- histochemical reaction of actin in both cochlear hair cells andsupporting celIs were attenuate and the difference between normal ratswas very great (P<0.0l); the difference of relative content of actin incochlea at different iron-deficient period was not significant (P>0.05). |
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