Prokaryotic Expression And Application Of CSFV Protein C, NS5A And NS5B

In the study,Recombinant proteins C,NS5A and NS5B of CSFV were expressed in E.coli expression system.The result of western blot showed the three proteins could be well identified by CSFV positive serum respectively exhibiting good antigenicity. The achievement of proteins C,NS5A and NS5B provides good preparation for genetically engineered vaccine and the establishment of specific serological diagnosis, and related function study of the three proteins.Offered advanced technology for diagnosis of the disease of CSFV. Based on the purified recombinant proteins C,NS5A and NS5B as diagnosis antigens and the expression of empty vector as negative antigen, an indirect ELISA method for detection of CSFV antibodies was developed. And the conditions were optimized.Compared with CSFV-Ab test kit of IDEXX, the result showed:the best consistency is the C-ELISA.Methods Serum specimens from 302 pigs of different ages from 3 days to 16 weeks old pigs and sows, were tested by the indirect ELISA for specific antibody against recombinant protein C. Comparing with CSFV-Ab test kit of IDEXX pointed that sows serum testing consistency is higher than piglets. It is showed we can use C-ELISA to test the serum of sows. Comparison of ELISA method in two shows the duration of CSFV C antibody is less than E2, but the detection of the time is earlier than E2.