Analysis Of Genetic Diversity Of 9 Chinese Indigenous Domestic Geese Based And Effect Monitoring Of Allopatry Conservation

Combining the technique of PCR and the fluorescent automated detection, as a platform on an ABI 3730xl genetic analyzer (Applied Biosystems) and GeneScan-500 as size standard. Based on existing research reports, selected 10 pairs of polymorphic microsatellite markers. Genetic diversity and phylogenetic relationship among 540 individuals of 9 Chinese domestic conservation goose breeds (Wanxi White goose, Zhedong White goose, Sichuan White goose, Taihu goose, Shitou goose, Huoyan goose, Wugang Tong goose, Magang goose and Wuzong goose) which from National Waterfowl Germplasm Resource Pool and two origin conservation goose breeds (Wanxi White goose, Shitou goose) which from Anhui and Guangdong province were evaluated with 10 microsatellite loci. Alleles were scored using GeneMapper v4.0 software (Applied Biosystems). The genetic diversity was disclosed by calculating the allele frequency, heterozygosity (H), effective number of alleles, polymorphism information content (PIC) and Reynolds'genetic distance. Phylogenetic Relationship was analyzed by the neighbor joining method (NJ). The genetic variability within populations and genetic differentiation among populations were estimated, and compared two effect of breeds conservation. Our main results were summarized as follows:1. The 9 indigenous goose breeds with abundant genetic resources and have a large range of variation. A total of 89 alleles were detected in 9 populations with 10 microsatellite markers. Microsatellite loci have alleles from3 to 26, and the average number of observed alleles was 8.90±6.61. The overall expected heterozygosity of all populations and PIC of all loci were 0.5785±0.012 and 0.5949 respectively. Magang goose have the highest genetic variation.2. The results showed that, there were 15 private alleles and a large number of dominate alleles at the 10 microsatellite loci of 9 indigenous goose. Except that the locus of WWX1, CKW13 and CKW14, other locis have one private alleles at least and nine alleles were common alleles. We construction STR characteristic spectra of 9 populations with 10 microsatellite loci.3. The genetic differentiation among populations had reached to 0.112 (P<0.001), which was very significant for the whole population, all loci were contributed significantly (P<0.001) to this differentiation. Reynolds' distance values varied between 0.0454 (Sichuan White goose -Wugang Tong goose pair) and 0.2342 (Huoyan goose–Shitou goose pair). The Nm value was ranged from 0.7404 (between Huoyan goose and Shitou goose) to 5.3806 (between Wugang Tong goose and Sichuan White goose). The phylogenetic relationship based on DR among Chinese indigenous goose were analyzed, an un-rooted consensus tree was constructed, and the 9 indigenous goose and divided them into 4 groups.4. The detection results were compared and analyzed between automated fluorescent Capillary Gel Electrophoresis and Polyacrylamide Gel Electrophoresis (PAGE). The results showed that, Capillary Gel Electrophoresis has higher precision than PAGE in the analysis of STR.5. The genetic diversity measures were not significantly difference between 9 Chinese domestic conservation goose breeds which from National Waterfowl Germplasm Resource Pool and two origin conservation goose breeds. It confirm that the effect of allopatry conservation is feasible and The protection method can effectively keeping the characteristics of varieties of their own germplasm.