Molecule Detection And Identification Of The Insect Resistance On Transgenic Ramie (boehmeria Nivea L.)

Ramie (Boehmeria nivea L.) which has good fiber quality and is loved by general public is an important fiber material in our industy of bast fiber manufacturing and one of main export-oriented fiber materials in China. Ramie is easily damaged by many injurious insects during the growth of it, which has effects on its physiological-biological activities in the process of growth and development. Consequently it will influenced the field and quality and bring about huge economic loss to many cultivators. So incect-resistant cultivars is the key to the solvement of insect pest and the increasement of the field and quality. With the development of genetic engineering, genetic transformed methods can transform exogenous incect-resistant genes into the genome of ramie, and make them express and produce poisonous substances which can kill insect pests. In this paper, genetic transformed plants of ramie cultivar Zhongzhu No.1 obtained by our laboratory were detected and filtered, and insect resistance of genetic transformed plants of T0 generation were identified, which provide data reference to the in-depth study on genetic transformation of ramie and lay the foundation to the subsequent incect-resistant breeding. Main research resuts as follows:(1) Through the detection and filtration to the genetic transformed plants of T0 generation, a series of genetic materils that show positive by detection were obtained, and percent conversion of exogenous CpTI gene is a bit higher than that of exogenous CryIA gene. The integration of exogenous genes was variant and exogenous transformed genes can transcribe.The ratio of genetic transformation plants of ramie obtained by Agrobacteriumtumefaciens-mediated was 65.5%(2) The preliminary study of genetic stability of exogenous genes in transgenic plants of T1 generation indicates exogenous genes still existed, and the phenomenon of the lose of exogenous genes was also discovered. Those exogenous genes of genetic plants of T1 generation whose exogenous genes yet existed can transcribe.(3) The identification of incect-resistance to transgenic plants of T0 generation refered to three aspects: the degree of fondness and harm of pests to various transgenic plants, and the deadly situation of various transgenic plants to pests. The result accounted for that the degree of fondness of pests to various transgenic plants of T0 generation was different; insect resistance of various transgenic plants of T0 generation to pests was different: those that showed high-grade inect-infection and inect-infection were comparatively more; those that showed moderate incect-resistance were comparatively less; there was no plant that showed incect-resistance and high-grade incect-resistance. Compared with the control, transgenic plants showed insect resistance which was comparatively lower and did not meet the requests of insect–resistant cultivars.(4) The cluster analysis of three indexes of incect-resistance (AI, Emendatory mortality rate and Harmful index of leaves) indicated that the three indexes which was effective in the aspect of the analysis of incect-resistance of T0 generation can be reference to the subsequent in-depth study.