The Influence And Effection Of Biomass On Soybean Symbiosis

In this experiment, as tested chernozem soil, using outdoor pot experiment, field experiment, the analysis method of indoor testing several different biomass of indigenous rhizobia, arbuscular mycorrhizal fungi in symbiosis with the soybean and related indicators effects. Main results are as follows:1. By looking at straw, potato skins, edible waste material, soybean extract liquid was applied to four types of biomass effects on soybean field experiment double symbiosis, we found that in V5 of straw significantly increased the number of AMF spores and fungi in rhizosphere soil number, to the R3 phase, AMF spore number of rhizosphere soil fungi, AMF infection rate, root soluble sugar content reached the maximum and higher than others, R6 significant increase in the number of effective nodules and reached its maximum rhizosphere soil increase in the number of bacteria, plant dry matter accumulation of more and also significantly increased soybean yield, biomass at the four best.Extracts of soybean processing in the whole growing number of effective nodules, root AMF colonization rate, AMF spores were significantly higher than the control soil, but the output is not high, indicating that the double symbiosis of soybean leachate have stimulated a relatively low yield is due to seed soaking before sowing lead. Mushroom potato skin waste material handling and processing only in the R6 of AMF infection rate was significantly higher than other treatments.2. A field experiment of straw and different fertilizer effects on soybean double symbiosis found that straw group effective nodule number, effective nodules, root AMF colonization rate and the AMF spores in soil were significantly higher than those without straw group, that straw on the dual symbiotic system is very beneficial. Fertilization factors reflected in the low fertilizer fertilizer, high fertilizer more conducive to effective nodules, nodule weight effectively, AMF colonization rate, AMF spore numbers increase, so the right amount of fertilizer will help double the establishment of the symbiotic system. Six treatments, without straw group, double symbiosis and fertilizer on soybean yield of soybean is significantly better than low-fat and high fat.the fertilizer with straw on the growth of nodule formation was significantly stimulated.Effective nodule weight, AMF colonization rate, spore, soluble sugar content, have a certain role in promoting. High fertilizer treatment on the effective nodules with straw weight and number of AMF spores are inhibited, which is due to the high nitrogen fertilizer than the fixed limit of straw to inhibit soybean symbiotic development and function of the play, thereby affecting the yield increase.3. Through the outdoor pot experiment, examine the straw, imbibed germinating temperature, processing liquid (water, DHG, DHG crude extract, pure genistein) three factors on soybean symbiotic system of double and effect. We found that straw in effective nodules, AMF infection rate of AMF spores in soil, soil bacteria, soil fungi, nitrogen, phosphorus content, plant height, seed number per plant and yield per plant were significant indicators or very significantly higher than those without straw.Imbibition of low temperature on soybean germination growth, nodule formation, AMF spore number in soil, plant nitrogen, phosphorus, plant traits and yield are inhibited, but the number on the soil, can promote the role of potassium. Four kinds of treatment solution and pure DHG and the role of double symbiotic best, significantly increased the number of effective nodules, AMF infection rate of AMF spores in soil and thus increase the number of grains per plant, yield, consider the pure expensive propose to use the economical and effective to improve soybean traits DHG and yield.