Develop An Animal Model Of Liver Cirrhosis In Bama Miniture Pig And Research Its Succession Of Intestinal Flora

BackgroundLiver cirrhosis is an important ailment that imperil the health of human, the ailment of liver and liver cirrhosis which caused by many factors has became more and more, so it was a hotspot of digestive disease. The only way to cure liver cirrhosis and telophase ailment of liver is liver transplantation, but it kept lower rate to make a successful operation, moreover immunotherapy, surgical skill and some other diseases cause by operation need further more research, so it is necessary to develop a large-animal models of liver cirrhosis to solve problems above.Bama miniture pig is a specific laboratory animal in our country, with lots of characters such as high degree of inbreeding, stability heredity, small size, besides Bama miniture pig habit of feeding, food combining, digestion, physiology and intestinal flora, its anatomical structure also as similar as human, so compare with rats and mouse, the Bama miniture pig is more suitable to be used to simulate disease of human, research transplantation of organ, surgical operation, intestinal microecology and so on.There are lots of microbial flora inhabit animal and human intestinal, and format a relative dynamic equilibrium and stable micro-ecosystem with intestinal environment, from the digestion and absorption, immune disease affects so many aspects of health. In recent years studies have shown a variety of diseases such as diabetes, metabolic syndrome, chronic malnutrition, cirrhosis and so on, their occurrence and development often accompanied by changes of intestinal microflora in the structure, therefore, the changes in intestinal flora caused by the diseases become a hot micro-ecological studies.Because traditional microbiological techniques could only analyze the micro-organisms which can be cultured, it exists flaws and shortcomings during studying microbial ecology system. Therefore, to accurately evaluate intestinal flora changes and succession laws need to develop other methods. The denaturing gradient gel electrophoresis (denaturing gradient gel electrophoresis, DGGE) technology based on 16S rDNA which intuitive, fast and high-throughput, it can reflect and analyse effectively the complex microbial flora. At the same time,16S rDNA is a way to get rid of traditional method which analysed environmental microorganisms by thoroughbred training, presently, both has been widely used in research related intestinal microflora.purposes and significanceBama miniature pig as experimental animal, by injecte exogenous hepatotoxins (CCl4 mix olive oil) supplemented diet control, build a experimental animal model of liver cirrhosis which stable, reproducible, convenient and strong simulative, to study the succession of the intestinal flora which change by the process of cirrhosis, and to explore relations between intestinal microflora and the occurrence and development of liver cirrhosis, and provide experimental basis data and reference for related diseases and micro-ecological studies.Methods1. Inject 40% solution which mix CCl4and olive oil at abdomen, with low protein and high carbohydrate diet, while 10% of ethanol as the only beverage.2. By PCR-DGGE Analyse changes of intestinal flora in Bama miniture pig and succession laws during the process of forming cirrhosis model.3. Viable countResults1 Bama miniature pig liver cirrhosis model was successfully prepared. Experimental group,10 Bama miniature pigs (6 male 4 female), from 3 mouths old began modeling miniature pig model of cirrhosis with complex way, compared with the control group the appearance of their size, weight and liver function are different, the liver was deteriorating condition, HE staining showed molding Bama miniature pig hepatic lobule is not continuous, and surrounded by collagen fibers, widely distributed pseudolobule severe deterioration of liver cells, fatty necrosis.2 Intestinal flora changed significantly during the preparation of Bama miniature pigs liver cirrhosis modelThe type, quantity and structure of intestinal flora in the control group basically stable, The experimental group compared with the control group during construction of the model, the number, type and complexity of bacteria have significant changed, the number of bacteria increased markedly, the bacteria grow excessively during the end time, disturbance of flora.