The Research On The Analgesic Effects And Mechanism Of Lappaconitine Following Operation In Dogs

Objective:This study was designed to reveal the analgesia mechanism of lappaconitine according to the evaluation the analgesic effects and contrast of analgesia mechanism,in order to provide reference of clinical application.Method:18 clinical healthy local canines were divided into 6 groups: Group A,Group B,Group C,corresponding lappaconitine 2mg,4mg,8mg once only;Group D——tepoxatin(20mg/kg,once only),Group E——non-analgesic,Group F——blank.The ovariohysterectomy was selected as postoperative pain model(exept group F),at preoperative time,operation completed time,2h postoperative,6h postoperative,12h postoperative,24h postoperative,rectal temperature,respiratory rate,heart rate,pain scores and peripheral blood(for determination of plasma concentration of PGE₂) were collected for statistical analysising.Result:There was significant change of rectal temperature in lappaconitine groups(P＜0.05),while respiratory rate,heart rate were not(P＞0.05);There was no significant change of pain scores between group D and group B(P＞0.05);the postoperative plasma concentration of PGE₂ in group E was increasing significantly as time went.The using of lappaconitine was able to significantly decrease plasma concentration of PGE₂(P＜0.05),it was most effective in group B;the inhibition of PGE₂ was extremely significant in group D(P＜0.01),and without significant difference between group B and group D;there was no significant changes of plasma concentration of PGE₂ each time in group F,Conclusion:Lappaconitine is as effective as tepoxalin but cheaper,the dosage 4mg per-time used for subcutaneous injection is most effective,but further research of precise dosage is needed.The inhibition of PGE₂ is additional analgesia mechanisms of lappaconitine.