Effects Of A Combination Of TSF, PGRF Gene Plasmid And SeY On Growth In Weaned Piglets

The objective of present trial was to investigate the effects of TSF, TSF combined with pGRF or Yeast Se on growth and the capacity of diseases resistance. Two experiments were carried out:Exp1: A 28 day experiment was conducted to evaluate the effect of Tremella fuciformis Berk Spore Fermentation (TSF), the combination of TSF with pGRF(porcine growth hormone releasing factor), the combination of TSF with pGRF and yeast selenium on growth, immune and intestinal microflora of weaned piglets. Thirty 28±2d weaned DLY (Landrace×Yorkshire×Duroc) piglets were randomly allocated to 5 treatments with 6 replicates of 2 piglets according to gender and body weight (BW), one treatment was act as control group feed on basal diets, and the other four treatments were feed on the same basal diet supplement with antibiotics (kitasamycin + kangdisu sulfas), TSF (4000mg/kg), 4000mg/kg TSF combination with intramuscular injection of 1mg pGRF into hind leg at the beginning of the trial, and 4000mg/kg TSF and 0.3mg/kg yeast selenium with 1mg pGRF intramuscular injection, separately. The results showed the TSF had the similar effects on the growth performance of piglets compared with antibiotics supplement. Both TSF and antibiotics supplement significantly improved the Average Daily Gain (ADG) and Feed: Gain(F/G) ratio compared with that of the control(p<0.05).The TSF+pGRF and TSF+pGRF+SeY groups showed a tendency to further improve the growth performance of piglets compared with that of the other treatment groups. The piglets supplemented with TSF ,TSF+ pGRF and TSF+ pGRF + SeY demonstrated a higher T lymphocyte transfer ratio (LTR) (P<0.05), serum IL-2 (p<0.05) and Classical Swine Fever (CSF) antibody titer compared with that of the control and antibiotics groups. Meanwhile, the number of T lymphocytes were also improved (P=0.10). Both TSF supplement groups significant increased the numbers of intestinal Lactobacillus and Bifidobacterium in piglets compared with that of antibiotics group (p<0.05), but showed no difference when compared with the control (p>0.05).the injection of pGRF showed no further improvement effects on immunity and intestinal microflora of piglets. In conclusion our results showed TSF supplements had siminal effects of promoting growth performance with antibiotics, and TSF can effectively improve the immune and optimize the intestinal microflora in weaned piglets. The combination of pGRF and TSF showed a tendency of further improving growth but had no impact on immune and intestinal microflora of weaned piglets.Exp2: The TSF+pGRF were chose based on the previous results in Exp1 to evaluate the effect of TSF combination with pGRF on disease resistance of weaned piglets. Fifteen 65±3d TaiHu piglets were randomly assigned to three dietary treatments with 5 replicates of 1 piglet according to weight and gender. The three treatments were as following: control group (fed on basal diet), antibiotic group (basal diet supplemented with kitasamycin 40mg/kg and dandisu sulfate 20 mg/kg), and TSF+pGRF group (basal diet supplemented with TSF 4000mg/kg and intramuscular injection of 1mg pGRF into hind leg at the beginning of the trial). At the beginning of the trial, the piglets were inoculated with vaccine against CSF and at the day 28 of the trial, the piglets were inoculated with virulent of CSF. the growth performance, disease resistance, and immunity of piglets were measured. The trial lasted for 35 days. The results showed TSF+pGRF enhanced the resistance for CSF virulent and shortened the altithemal and pathogenesis period, reduced the maximum of the body temperature and elongated the latency period. Compared with the control group, the TSF+pGRF or antibiotics significantly (P<0.05) improved the antibody titers of serum for CSF, and highly significantly elevated the concentration of serum IgG in piglets (P<0.01). TSF+pGRF significantly (P<0.05) increased the average daily gain and feed efficiency when compared with the other two groups, but there were no difference of average daily gain and feed efficiency during the third to forth week of the trial. In conclusion, TSF+pGRF could enhance the disease resistance, immunity and growth performance of the piglets.