Studies On Transformation Of Tomato With Vaccine Of Recombinant M2 Peptide Of AIV And Keratinocyte Growth Factor

Avian influenza virus(AIV) infect almost all wild birds and domestic poultries and then decimate poultries.Severe economic loss of the poultry industry was brought because of the AIV prevalence.The M2 protein of AIV is a kind of cross-protection constructed by 97 amino acids,and a high evolutionary conservative protein in different subtypes.Because AIV infects animals by the respiratory and digestive way,it is important that this kind of AIV vaccine can lead to animal's mucosal and system responses.Keratinocyte growth factor,composed of 163 amino acids,is one of the cell Mitogen-activated factors.KGF plays an important role in the growth and rebirth of epidermal cell.It can accelerate the bladder,nephridium,intestines and corneal epidermal wound healing.It can also alleviate the chemotherapy and irradiation induced injure.Transgenic plants that express foreign proteins with industrial or pharmaceutical value represent an economical alternative to fermentation-based production system. Tomato(Lycopersicon eseulentum),one of the spread-widest planted vegetable,is a kind of rich nutrition plant.It is so valuable to use tomato to produce Vaccine of Recombinant Peptide of AIV and KGF.The study focuses on the effects of different concentration of 6-BA,zeatin and IAA on tomato cotyledon regeneration.And also the tomato regeneration system is improved. The result indicates that the normal regenerating shoot ratio is the highest(36.37%) when the adding zeatin concentration is 0.5 mg/L.A total of 34 putative transgenic tomato plants with LBM2eHBc+ gene are obtained and it is proved that the LBM2eHBc+ gene has been inserted into these tomato genomes by PCR.The gene KGF is also inserted into pB1121 vector and then transformed into tomato.It is proved that the gene KGF has been integrated to the tomato genomes by PCR.