The Establishment Of Sterilized Seedling Culture System Of Solidago Canadensis And Study About Chemical-ecological Effects Of Solidago Canadensis Root Extracts

Solidago Canadensis was introduced from Japan and cultivated as garden flowers in Shanghai, Nanjing area in 1935, considering as high risk and harmful weeds in 1980s because of its proliferation, threading to the species diversity of ecosystems where was invaded. Solidago Canadensis has become the East China the most common environmental weeds and most seriously harmful alien invasive species, the problem about wanton growth of Solidago Canadensis is increasingly outstanding. To this end, this study was based on hypothesis about "allelopathy in growth process of Solidago Canadensis", by the establishment of sterilized seedling culture system of Solidago Canadensis in order to avoid complexity and uncertainty of qualitative and quantitative analysis of plant roots extracts in soil. By simulation Solidago Canadensis seed germination and seedling growth conditions with the help of artificial climate box, we carried out biological assay about the effect of aqueous extracts, n-butanol extracts and remaining aquatic extracts to Triticum aestivum, Aeschynomene and Solidago Canadensis, revealed allelopathy of Solidago Canadensis. On this basis, we analysed n-butanol extracts by silica gel column chromatography and high performance liquid chromatography, and further identified that root exudates Solidago Canadensis have significantly allelopathy. Research results are as follows:(1)At a temperature of 25-30℃, added 6-BA 5.0mg / L, NAA 0.5 mg / L ,2,4-D 1.0 mg / L, IAA 0.1mg / L under the conditions of the medium suitable for the establishment of aseptic seedling of Solidago canadensis culture system bacteria.(2)In seed germination experiment, the effect, aquatic extracts, n-butanol extracts and the water phase concentrate of Solidago Canadensis root on seed germination of Triticum aestivum, did not have a significant impact.(3)In seedling growth experiment, the effect, aquatic extracts, n-butanol extracts and the water phase concentrate of Solidago Canadensis root on seedling growth of Triticum aestivum, Aeschynomene and Solidago Canadensis itself, was significantly inhibited, in which the effect of n-butanol extracts on seedling growth of Triticum aestivum, as well as Aeschynomene and Solidago Canadensis are the strongest inhibitory of all. N-butanol extracts significantly inhibited effect on seedling growth of Triticum aestivum, Aeschynomene, and Solidago Canadensis at a relatively low concentration at 1.0mg/mL, while at a relatively low concentration (0.5mg/mL) as a catalytic role.(4)The effect about three products isolated by column chromatography of n-butanol extracts from of Solidago Canadensis root on seed germination was not significant, and on the growth of Triticum aestivum seedling allelopathy had a concentration effect. No.Ⅰseparated product had the most significant inhibition effect on the growth of Triticum aestivum seedlings, and at a relatively low concentration (0.5mg/mL) inhibited the growth of wheat seedlings significantly.