Hereditary Change Analysis Of Porcine Reproductive And Respiratory Syndrome Virus GP5 Gene In Chongqing Partial Area

Porcine Reproductive and Respiratory Syndrome(PRRS) are at the end of 1980s discovery one kind of altitude communicable disease,causes pregnancy reproduction barrier symptoms and so on sow's miscarriage,still birth,mummy embryo,as well as each kind of age pig(is specially newborn pig) the respiratory tract symptom,its cause of disease drafts the virus for Porcine Reproductive and Respiratory Syndrome Virus(Porcine Reproductive and Respiratory Syndrome Virus, PRRSV).This research originates in the Chongqing area 8 clinical doubtful sickness material took the object of study,the distinction naming is T1,H1,B1,C1,B2,C2,H2,C3,according to a Chinese standard sequence,designed six pairs using software Primer5.0 to aim at the GP5 gene the specificity to direct the thing,the distinction naming is P1.1/P1.2,P2.1/P2.2,P3.1/P3.2,P4.1/P4.2, P5.1/P5.2,P6.1/P6.2,finally used to direct thing P6.1/P5.2 to expand through RT-PCR increases 777bp the gene fragment,its insertion clone carrier material particle PMD19-T.The construction reorganization material particle,carries on the sequence determination to the clone GP5 gene.The experimental result indicated T1,B1,C1,B2,H2,C3 this 6 are the masculine genders,8 sample masculine genders pick out rate are 75.0%.C3 has not been able to determine the sequence, measured the foreword result showed,T1,C1,B2,H2 GP5 gene span 603bp,contains the integrity the GP5 gene opening reading frame,codes 200 amino acids,B1 GP5 gene span 604bp.5 poison GP5 gene sequence obtains which this research carries on the homology comparison, finally demonstrated 5 poison between the nucleotide sequence homology is 96.2%～99.8%,T1 and B1,T1 and C1,T1 and B2,T1 and H2,B1 and C1,B1 and B2,B1 and H2,C1 and B2,C1 and H2, B2 and the H2 homology respectively is 97.7%,97.7%,98.5%,99.8%,96.2%,97.5%,97.5%, 97.7%,97.5%,98.3%.Carries on the homology this 5 with the VR-2332,JX-1,JXA1,Henan-1, NX06,BJsy06,HB-1,CH-1a,LV GP5 gene sequence to compare,finally demonstrated 5 poisons and the VR-2332,JX-1,JXA1,Henan-1,NX06,HB-1,CH-1a,LV GP5 sequence homology respectively is87.9%～89.4%,96.7%～98.2%,97.7%～99.5%,98.0%～99.3%,97.8%～99.7%, 95.5%～97.0%,94.0%～96.2%,64.5%～65.6%.Except that between the B1 its inferential reasoning code amino acid sequence homology is 97.0%～99.5%,in which T1 and C1,T1 and B2,T1 and H2,C1 and B2,C1 and H2,B2 and the H2 homology respectively is 97.5%,97.5%,99.5%,98.0%,97.0%,97.0%.With other domestic and foreign poison similarity is 59.5%～100.0%,in which H2 and the LV homology most is low is 59.5%,in which B2 and NX06 and the BJsy06 homology is 100.0%.Indicated domestic popular poison is small in the gene region variation,confirmed 5 poison are the Americas.The inferential reasoning code amino acid comparison result indicated that,the neutral epi-position and the non-neutral epi-position region has the variation,(13 aa and 151 aa) does not have the variation with the toxicity correlation amino acid.With domestic and foreign 20 PRRSV corresponding sequences which publishes has carried on the comparison 5 popular poisonous,and has established the heredity cladogram.The result indicated that,PRRSV poisonous divides into 2 genotypes(the Americas with Europe),the Americas may divide into 4 subgroups.In this research obtains popular poisonous all belongs to the AmericasⅠSubgroup.