Study On Tissue Culture Of Fritillaria Unibracteata Kisao Et K.C.Hisa

In this paper,differentiation of bulb of Fritillaria unibracteata Kisao et K.C.Hisa from adding a combination of different hormones of NAA and 6-BA.the results that the best bud induction medium:MS+NAA1.0mg/L+6-BA 0.5mg/L,Generated the largest number of adventitious buds and shoots are more robust.Proliferation of adventitious bud the best medium:MS+NAA1.0mg/L+6-BA 1.0mg/L;Buds grow more quickly,sturdily,evenly。At the course of the study Fritillaria unibracteata Kisao et K.C.Hisa tissue culture,The GA of Fritillaria unibracteata Kisao et K.C Hisa formation of adventitious bud also plays a role,One of the physiological role of GA are able to effectively remove the plants dormancy,To promote germination observably。So in this experiment GA breaking Fritillaria unibracteata Kisao et K.C.dormancy and shortens of bulbs differentiationAt the course of the study Fritillaria unibracteata Kisao et K.C.Hisa tissue culture,Besides the combination of different hormones influence Fritillaria unibracteata Kisao et K.C.Hisa in adventitious bud formation,Explant selection of site,temperature,light,sucrose concentration conditions also affect the results of the experiment,When the temperature is 20℃,8h/d light,2000Lx light intensity and sucrose concentration of 5%of the adventitious buds generated the largest number,and the buds are more robust,The same scales in different parts of the differentiation capacity of different:Scales easier to divide the base,23d can be about a small bud differentiation,differentiation of buds more(some can be differentiated adventitious buds 4-5 months) of bud differentiation than Armstrong;scales at the top of differentiation budding 30d required around,and differentiation of buds and fewer buds 1-2) and some even do not divide.Valuable for the study of medicinal plants -Fritillaria unibracteata Kisao et K.C.Hisa both value and practical value,the study can be many sterile bulblet,on the one hand,to obtain substantial bulblet research materials,on the other hand,for the realization of large-scale breeding of laying the theoretical and experimental foundation. The study of medicinal plants resources,introduction and domestication and the development and utilization of theoretical and practical significance.