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Study On Filtration Of Food Attractant And Their Mixed Ligand Of Lasioderma Serricorne In Room

Posted on:2009-08-07Degree:MasterType:Thesis
Country:ChinaCandidate:W XiongFull Text:PDF
GTID:2143360248451635Subject:Agricultural Entomology and Pest Control
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Cigarette beetle Lasioderma serricorne(F.), belongs to Coleopter: Anobiidae. one of the most notorious storage pests, It is so well-known for its wide adaptability, strong propagation and good appetite of various foods that you can find it nearly at the every corner of the world. It can do harm to a lot kinds of hosts, especially cured tobacco strip leaves. In this study, we had chosen tobacco beetle as target pest and its common hosts to study tobacco beetle's preferable host. Next we selected the preferable hosts and distilled their odor volatiles to test their attrctive activity separately. And then we mixed the extracted odor volatiles with different doses to study the mixed ligand activity and food attractant enhanced effect of sex attractant for Cigarette beetle.1 Preference of the cigarette beetle to different host foods and plant-oilsTaxis responses of L.serricorne female adults to 26 host foods were measured using a Y-tube olfactometer in the laboratory. The results indicated: compared with the control, the female adults of L.serricorne were attracted significantly by 21 host foods and were attracted very significantly by 14 among of 21 host foods, the relative attractive rate were 25-75%. the others 5 host had no significantly attractive effect on the female adults. taxis responses of L.serricorne female adults to six plant-oil Using a two-choice pitfall in the laboratory on 5μl dosage. The results indicated: the female adults of L.serricorne were attracted significantly by only two plant-oil: sesame oil and rapeseed oil, the relative attractive rate of sesame were slightly higher than rapeseed oil, it was 37.5%.2 Attractive activity of the cigarette beetle to 5 host food volatiles and sesame oilThe materials above were classify by six sorts, and chose one or two meterials of high relative attractive rate from each sort for extraction of volatiles. Behavioral responses of L.serricorne female adults to 5 host food volatiles and sesame oil were measured using a two-choice pitfall in the laboratory. The results indicated: The relative attractive rate of volatiles and sesame oil became lower as the concentration decreased. When dosage was below 0.05μl, there were no significant difference between treat and control. The relative attractive rate of most volatiles was significant decrease when the dosage was down to the 0.5μl , but the citronella green tea and sesame oil were 0,05μl. the relative attractive rate of citronella on 5μl dosage was more than 50μl. the most of relative attractive rate of all material were 40-60%. the highest relative attractive rate was 58.75% on 50μl of green tea and 5μl of fennel.3 Attractive activity of the cigarette beetle to host food volatiles and sesame oil mixed in pairBehavioral responses of L.serricorne female adults to 5 host food volatiles and sesame oil mixed in pair on 5μl, 0.5μl, 0.05μl dosage were measured using a two-choice pitfall in the laboratory. The results indicated: The relative attractive rate of mixed precriptions became lower as the concentration decreased. When dosage was below the 0.05μl, there were no significant difference between treat and control except citronella+alfalfa and fennel+green tea, the amplitude of decreased also were low, further research in 0.005μl, find that there were no significant difference between treat and control, relative attractive rate decrease significant. Comparison of relative attractive rate of each mixed precriptions and its composing on 3 dosage. The results indicated: only relative attractive rate of citronella+alfalfa was no less than the composing which were citronella and alfalfa on every dosage.4 Study on synergia effect of food attractant to the sex pheromoneSelect 4 food attractant from the above results. Behavioral responses of L.serricorne adults to the 4 food attractant combined with the serrico were measured using improved two-choice pitfall in the laboratory which stimulated tobacco storage. The results indicated: The relative attractive rate of the 4 food attractant combined with the serrico were more than single serrico ,there were significant difference between the food attractant combined with serrico and serrico, the same results to the female adults. The sex proportion of entrapped pest adults was as following: food attractant combined with serrico were about 0.5 (female/male); single serrico was about 0.3 (female/male).
Keywords/Search Tags:Lasioderma serricorne(F.), host volatiles, relative attractive rate, mixed precriptions, synergia effect
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