Bio-effects Of Ferula Ferulaeoides (Steud.) Korov. By N~+ Implatation And Tissue Culture

In this paper, the seeds were implanted by the different doses of N⁺ and the germination percentage and germination index were researched, the highest germination percentage was obtained in the doses of 1×10¹⁶ cm^-2,and the germination percentage of which was 45.55±5.66%. The results showed that for Ferula ferulaeoides, low-doses of N⁺ beam implantation could be effective in breaking dormancy and enhancing the germination percentage. The effects of N⁺ implantation on several physiological and biochemical Indexes of Ferula ferulaeoides Seed were studied. Those results illuminated that the activity of SOD, POD and CAT were enhanced with the increase of the doses. However, excessive dose of nitrogen ion beam irradiation reduced the activities of SOD, POD and CAT. The proline content was decreased, but the proline content was significantly increased at the dose of 6×10¹⁶ ions / cm². Besides, the soluble protein content was increased with the increase of the dose, the soluble protein content was descend at the high dose of nitrogen ion beam irradiation. The soluble protein content raised first and then decreased, the dose 6×10¹⁶ cm-2 reached the highest. After the different doses of N⁺ implantation, as the dose increases, MDA showed the trend that is lower then higher and finally lower too. There was a marked increase at the dose of 3×10l6 cm-2. Through experiments contrast, the results showed that the ferulic acid content of Ferula ferulaeoides of the Asafetida was significantly lower than Ferula sinkiangensis asafetida. The ideal medium that induced leaf and stem formed callus were gained. Containing 0.3mg/L NAA and 1.0mg/L 6-BA, the inducement rate of leaf callus was 75%. Containing 0.5mg/L NAA and 0.5mg/L 6-BA, the inducement rate of stem callus was 81.25%. The best Callus Medium for the proliferation contained 2.0mg/L 2,4-D and 1.0 mg/L 6-BA, and the inducement rate was 100%. This experiment showed that in some extent the higher concentration of growth hormone and lower concentrations of cytokinin were good to the callus induction and proliferation, it also noted that it is difficult to carry out the Ferula ferulaeoides leaf with the tissue culture, and the stem is the appropriate tissue culture material.