The Development Rule And Relativity On IMF Content Of H-FABP And PPAR-γ MRNA Expression Level In Tan Sheep And MengGu Sheep Muscle

In recent years, the consumption of meat in the world is improved swiftly and violently, and the quality is more regard than quantity. So the production of stockbreeding should improve for this demand, to adjust the need of Social and Economic benefits.To improve the situation about low quality in sheep mutton, the study is designed for the direction of express level about mRNA of H-FABP and PPARγof intramuscular fat acid content. The Rule and Relativity on IMF Content of H-FABP and PPAR-γmRNA Expression Level are detected, and hope to provide some basic research for the future.12 Tan Sheep and 12 Meng Gu Sheep breeds in same condition were killed on Day 90, Day 120, Day 160 and Day 200. The level of expression of H-FABP and PPAR-γin Longissimus dorsal muscle was detected by the method of Semi-Quantitative RT-PCR Assay, and the IMF content was detected to study the rule between the growth and the relativity of the IMF content at the expression level of gene. The results as follows:(1)The IMF content in Tan Sheep of the second period was higher than the first, and no obvious change later then, the first period shows significant differences to the others(P<0.05);(2)The IMF content of Meng Gu Sheep was ascend with the days, but there is no significant differences between 4 periods;(3)The expression of H-FABP in two breeds was ascended with days until Day 160, and then it began to go down. The expression of Tan Sheep on Day 160 takes a significant difference to the first and the second periods. The expression of Meng Gu Sheep on Day 160 was significant differences to the other 3 periods;(4)There is no obvious rule in the expression level of PPAR-γof Tan Sheep. It was highest on Day 160, which showed significant differences to the other periods. The Meng Gu Sheep was declined with the time until Day 160, and began to descend;(5) In Meng Gu sheep, the mRNA expression level of H-FABP was highly positively correlated (r=0.988, P=0.068) with IMF content from Day 90 to 160, but that of PPAR-γwas highly negatively correlated (r=-0.8849, P=0.1084) with IMF content in the same period.