Study On The DNA Methylation Patterns And Level Of Rice (Orzya.Sativa.L.) Treated With Napthalene

The phenomenon of variation refers to genetic and/or epigenetic changes that as a response to the environmental stress in plant material has been identified abroad. DNA methylation as an important epigenetic modification plays an important role in controlling gene expression and maintaining genomic stability in animals and plants. In this study, two elite rice inbred lines, Nipponbare and Mastumae were selected and were irrigated with naphthalene solution of different concentrations. Epigenetic instabilities in the blade of the plants in tillering and heading stage were assessed by MSAP marks. The main results obtained in this study were summerized as following:1. Genotype had an important effect on the ability resisting to the stress of naphthalene exposure. The fastness of Nipponbare is stronger than Mastumae.2.Naphthalene exposure induced epigenetic (based on different levels and patterns of DNA methylation) variations in all the samples.3.The linear correlation between the concentration and the level of DNA methylation has not been found. Whereas the blade in tillering stage has a higher DNA methylation level than in heading stage.4.The variation of DNA methylation does not have unified patterns. In general, the frequencies of demethylation (0.48%-10.41% ) were higher than hypermethylation (0.10%-1.92%).