Genetic Improvement And Properties Of High-iron Transgenic Rice Expressing A Pea Ferritin Gene

Using transgenic rice lines(Fer34,Fer65) and its control rice xiushui 11 as materials, the effects of different ecological environments and foliar application of ferrous sulphate on the iron contents of transgenic rice were investigated. At the same time, the low phytic acid mutant gene and ferritin gene were polymerizationed. The main resutlts were as followings:(1) Under the different ecological environments, transgenic rice lines (Fer34 and Fer65) had a stronger capacity of accumulating the iron than the control, but the high iron contents were dramatically affected by the growing environmental factors such as soil and climate.(2) The iron contents of the transgenic rice line (Fer34) was affected by the foliar application of ferrous sulphate.The results indicated that the iron content of Fer34 was higher than the control when foliar application of ferrous sulphate was carried out once or twice, early period or late period.(3) We carried out the anther culture of hybrid progenies between two high iron transgenic rice lines and two low phytic acid mutant lines, and evaluated the regeneration efficacy and several seedling traits including ploidy, survival rate and seed-setting. The results indicated that the low phytic acid gene and ferritin gene had a slight impact on the process of anther culture. By screening numerous progenies, 31 homozygous lines co-expressing low phytic acid gene and ferritin (Fer) gene were obtained after seeds GUS detection, leaves PCR analysis and Inorganic phosphorus analysis. Further analysis indicated 4 representative progenies lines had significantly high iron contents with 40%-50% higher than the control, indicating that they were elite low-phytic acid and high-iron breeding germplasm.(4) Choosing four representative progenies lines which had significantly higher iron content and its control as materials. The biological characteristics of pure lines co-expressing ferritin gene and low phytic acid gene was carried out.The results showed that, the germination days of pure lines were of obviously delayed, the young seedlings grew slowly in early stage, and the rate of early filling stage was not as fast as control. Although the differences gradually became smaller in the growth process, the final grain yield may be affected.