Screening And Primarily Analysis The Differential Expression Genes Related To The Development Of Goose Fatty Liver

In the goose fatty liver forming process, a series of changes were found in the shape and composition of the fatty liver, and the proteins were the material base of the liver change. 12 Landes geese at the age of 110 days were divided into two groups and 6 geese in each group. The overfeeding group were feed the high energy corn and the control group were feed normal ration, after 18 days feeding, the livers of two groups were taken by slaughtering, the liver differential expression protein between the fatty liver and the normal liver were screening by proteome method. The main results are as follows:(1) The test showed that the sensitivity of protein detection by Schevchenko, Blum and GE method were 50ng, 5-50ng and more than 5ng respectively. sensitivity of the classical Coomassie brilliant blue was between 0.05 and 0.5μg.(2) The proteins from the fatty liver and the normal liver were separated by two-dimensional electrophoresis respectively. After GE silver-staining, the results showed that the protein from Landes geese liver concentrating at pI4-6 and 43.0KD-97.4KD.(3) 45 different protein points were obtained by PDQuest analysis of the 2-D Gel. Using the MALDI-TOF-MS analysis which was supplied by Matrix Science Company from Intent, and 11 useful protein points were found: Glutamate transporter, because of its lower expression level, the central nervous system was affected by the high amount of Glutamate. Phosphoglycerate mutase and Triose phosphate isomerase, its lower expression level in fatty liver showed that overfeeding geese needed less energy than normal geese. Glutathione S-transferase, its lower expression level in fatty liver showed that the fatty liver's ability in eliminating Lipophilic compounds and detoxificating was seriously reduced. Ornithine aminotransferas, its lower expression level in the fatty indicated that the ammonia density in the blood elevated, which might cause the overfeeding geese brain cell damage. GTP cyclohydrolase-I, its lower expresses level in the fatty liver showed that overfeeding geese's blood pressure and cholesterol might be higher than that in the control groups. Thioredoxin, its low expression level in fatty liver indicated that the lower liver cell hyperplasiaed during overfeeding period. Serine hydroxymethyrl transferase, its lower expresses level in the fatty liver indicate that it might make overfeeding geese gene different methylation and the function of DNA's reparation abnormal. Nucleoside Diphosphate Kinase, overfeeding geese's ability of controlling the tumor's production and metastasis would be weaker because of the low expression of this kind of enzyme. Apolipoprotein E, its low exprsssion level in the fatty liver might make the cholesterol and triglyceride be abnormal in transportation and metabolism.