| Adopting the tri-factor orthogonal design, this trial have studied the seed production ofLolium multiflorum Changjiang cv NO.2 based on 3 factors including different sowing rate, rowspacing and plant growth regulations PP333. The effect of PP333 to the lodging resistance, seedyield and quality was also studied in this trial. Hope to found some high seed yield plantingtechniques and provide a useful reference to the study and application of Lolium multiflorum seedproduction further. The results show:1. Adopting the tri-factor orthogonal design, established model based on field experimentsindicated that sowing rate is the first effective factor in terms of effect to Lolium multiflorumChangjiang cv NO.2 seed yield, and row spacing, spray PP333 are following. The optimum rangeof the factors were given: sowing rate 1.375～1.681 g/m2,row spacing 32～40cm and spray PP333288～410mg/kg, the boundary effect of sowing rate is maximal, the rowing spacing is followingand the spray is least. Under cut application, the optimum ranges of the factors were given: sowingrate 1.540～1.857g/m2, row spacing 35～42cm, spray PP333 310～415mg/kg, the boundary effect ofthe rowing spacing is maximal, sowing rate is following and the spraying PP333 is least.2. The seed yield was increased as the concentration of PP333 raised and decreased as the sprayingtime delayed. The seed yield was increased significantly by spraying 400mg/kg PP333 at one leafstage, the seed yield reached 2783.5kg/hm2, 22.31％higher more than CK. When theconcentration reached 600mg/kg, the seed yield began to decrease, the yield was 2018.4kg/hm2 atone leaf stage, lower significantly than CK. So spray 400 mg/kg PP333 at one leaf stage would helpto increase the yield of Lolium multiflorum.3. The lodging resistance of Lolium multiflorum was strengthened as the concentration of PP333increased and weakened as the spraying time delayed. When the concentration of PP333 were400mg/kg and 600mg/kg sprayed at one leaf stage, the lodging resistance was strongest, lodgingcoefficient respectively were 11.54 and 11.84, were lower significantly than CK (P＜0.05). 4. PP333 was help to increase the ear length and spikelet number of Lolium multiflorum. The earlength and spikelet number were most by spraying 400mg/kg PP333 at one leaf stage, ear lengthwas 45.6cm and spikelet number reach to 44, increased by 4.5cm and 5 respectively than CK.Meanwhile, the weight of leaf and culm were decreased as the concentration of PP333 increased,when the PP333 spraying concentration reached to 400 mg/kg and 600mg/kg at one leaf stage, theweight was decreased respectively compared with CK (P＜0.01). The affection of PP333 to tillerand fertile tillers was first increase then decrease as the concentration of PP333 increased, thenumber was most by spraying 400mg/kg. In addition, there were little relevance among PP333 andflorets per spikes and 1000-grain weight.5. In the seed quality test and evaluation, the change of germinative enerage and germinativepercentage were consistent, when the concentrations of PP333 were 400mg/kg they were 82％upward and 70％respectively. The concentration of PP333 would cause the undulation of relativeelectric conductivity, it was deduced as the concentration of PP333 increased. The highest electricconductivity was 82.8％(initial period of young spike100mg/kg), and the lowest was 77.3％(oneleaf stage, 600mg/kg). TTC changed between 74.9％and 77.4％, it was increased as theconcentration of PP333 increased. The TTC contents in the seed which was processed by PP333 washigher than CK, in which A3B1(one leaf stage, 600mg/kg) had the highest TTC contents that was45mg/ul 10mg/ul higher than CK. In addition, the earlier spraying time of PP333 the higher TTCcontents in the seed, and the activity of dehydrogenase correspondingly increased. So PP333 couldnot only enhance the seed yield but also could improve its quality of Lolium multiflorum.
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