The Effect Of Dietary Lysine Deficient On Intestinal Microflora, Digestive Enzyme And Immune Function Of Juvenile Jian Carp (Cyprinus Carpio Var.Jian)

This study was conducted to investigate the effect of dietary lysine deficient on intestinal microflora, digestive enzyme and immune function of juvenile Jian Carp (cyprinus carpio Var.Jian). 258 juvenile Jian carps with initial weight 15.98±0.02g were allocated to 2 treatments randomly, with 129 juvenile Jian carps in each treatment. Each treatment had 3 replications, feeding diets with lysine deficient or not , fed respectively for 60 days to determine the effect of lysine deficient on intestinal microflora and digestive function of Jian Carp. After 60-day feeding, a challenge trial was couducted by injection of Aeromonas Hydrophila (AH), then fed for 17 days to determine the effect of lysine deficient on immune function of Jian Carp.The results showed that: lysine deficient made Jian Carp become black and muscular dystrophy .Dietary lysine deficient decreased significantly or great significantly weight gain ( P＜0.05 ), feed intake ( P＜0.01 ), feed conversion efficiency ( P＜0.05 ), intestine weight (P＜0.01) , intestine length (P＜0.01) , intestine index ( intestine weight/body weight) (P＜0.01), activity of trypsin (P＜0.01) , activity ofchymotrypsin (P＜0.01), activity of gama-glutamyl transferase(γ-GT) (P＜0.01), activity ofcreatinkinase (P＜0.01), blood hematocyte counts (P＜0.01), serum lectin (P＜0.05) ,serum alexin C₄ (P＜0.05), serum acid phosphatase (P＜0.01) and serum sim--IgM (P＜0.01) of Jian carp. Hepatopancreas weight, spleen weight, pronephros weight, mesonephros weight, serum alexin C₃ and serum Transfetrin (P＞0.05 ) had no difference between two treatment in statistics. Bacteriological analysis results showed that the intestinal lactobacillus counts (P＜0.01) decreased with Escherichia coli (P＜0.01) and Aeromonas hydrophila counts (P＜0.01) increased great significantly when dietary lysine was deficient.In summary, lysine deficient resulted in juvenile Jian carp became black and muscular dystrophy; dietary lysine deficient degraded protein digestion through degrading ability of decomposing from basic amino acid binding site and aromatic amino acid binding site; dietary lysine deficient degraded significantly immune of juvenile Jian carp through degrading ability of identification, adhesion and bacteriolysis; dietary lysine deficient resulted in intestinal lactobacillus counts decreasing with Escherichia coli and Aeromonas hydrophila counts increasing great significantly.