The Study On The Technical System Of Tissue Culture And Cell Suspension Culture Of Malania Oleifera

Malania oleifera is a peculiar single-species and single-category plant in ourcountry. Its seed has abound oil, among which, z-15-tetracosenoic acid is considered tobe the ideal raw materials of muscone synthesizes, so Malania oleifera has quite greatvalue on exploitation and utilization. The study offer theoretical foundation for industrialmanufacture of tissue-cultured plants and for the producing z-15-tetracosenoic acid bycallus and suspension cell.This paper studies comprehensively on the induction of aseptic seedlings, theimmediate induction of bud, callus induction and differentiation from stems andhypocotyls, rooting and transplanting of tissue culture shoots, callus induction andmultiplication from albumen and the cell suspension culture etc. ofMalania oleifera. Thepreliminary experiments' results as follows:â‘ August is considered to be the best season to draw materials, in which thegermination rate is 80.00%, and the contaminated rate is only 36.67%. The suitabledisinfection method of seeding plant's stem is 75%ethanol 10S+10%NaClO10min+0.1%HgCl₂8min. The suitable disinfection method of kernel is 75%ethanol10S+10%NaClO10min+0.1% HgCl₂30min.â‘¡The optimum recipe of the induction of aseptic seedlings from embryo isMS+NAA0.2mg/L.â‘¢Every part of aseptic seedling from embryo and the stem with bud of seedlingscan immediately induct bud, among which, the best material is stem with cotyledonsaxillary bud, of which the optimum recipe is MS+NAA0.1+6-BA1.0+KT1.0mg/L. Theoptimum recipe of stem without top-bud, hypocotyls and stem with bud of seedlings are MS+NAA0.2+KT1.0mg/L, MS+NAA0.1+6-BA1.0+KT0.5mg/L and MS+NAA0.1+KT1.0mg/L respectively.â‘£The optimum recipe of callus induction of hypocotyls and stem areMS+2, 4-D2.0+NAA1.0+6-BA0.4+KT0.2mg/L and MS+2, 4-D2.0+NAA1.0+6-BA0.2+KT0.2mg/L respectively. The callus induced from stem can't differentiate. Theoptimum recipe of differentiation from hypocotyl's callus is MS+NAA0.2+ZT3.0+KT2.0mg/L. However, just a little of peak green callus can differentiate, and the growthof bud is so thin that can't survive.⑤The bottom of aseptic seedlings becomes expanded after 10 days, and then a littleof callus appears, and thin-root is produced after 60 days, The inducing of root byintermittent illumination.â‘¥The best transplant medium is sand, which the rate of survival is 80.00%.⑦MS is considered to be the best basic medium for callus induction from albumen.The induction rate and the optimum recipe of different plant is varied. In givenconsistency, 2, 4-D with high consistency coorperated with 6-BA with low consistencycan promote efficaciously the induction and propagation of calli. KT and GA₃ withsuitable consistency can promote the growth of calli, Added with glycin can enhance therate of induction, but the callus is ill-fitted to the cell suspension culture. CM is inhibitingfor callus induction from albumen. The optimum recipes of callus multiplication isMS+2, 4-D2.0+6-BA0.2+GA₃0.4mg/L.⑧The suitable culture condition are following: the basic medium is MS, thehormone combination is 2, 4-D2.0+6-BA0.2+GA₃0.4mg/L, the initial inoculationconcentration is 4g/100ml, the pH value is 5.8, the culture temperature is 28℃and thesucrose concentration is 40g/L.⑨The content of z-15-tetracosenoic acid in albumen,callus induction from albumenand suspended solids are following: 31%, 11.3% and 2.9 g/L.