In Vitro Selection Of Salt-resistant Mutant In Kenaf (Hibiscus Cannabinus L.)

Hibiscus canabinus, an annual nerbaceous bast crop, belongs to Hibiscus, mallowfamily. The kenaf bast is a kind of important raw material of textile, and the paperpulp of entire stem can substitute importing water pulp and is a kind of fine rawmaterial for papermaking. China is the most country of kenaf production. Althoughhaving a relative tolerance to salt, kenaf crops suffer from salt severely. Moreover,with the decrease of total cultivated land per cap and increasely contradiction forproduction area between kenaf and grain crops, cotton, kenaf crop certainly willturn its steps to more salted-soil, even more Severely salted areas. Therefore, it is verynecessary to improve its salt tolerance.In the present paper, leaves of released kenaf cultivars Q3 and 7804 were used asthe explants to induce warty of leaf spheroid, then they differentiated and selected byNaCl in vitro to select salt-resistant mutant. The paper provided a fine fundament forHibiscus canabinus germplasm recreation and breeding for new cultivars. Here werethe main results:1. The callus warty induce percentage of kenaf were highly related to the gene-type, the induce percentage of Q3 were higher than that of 7804 in NaC1 stress or noNaCl stress.2. The callus warty induce percentage of kenaf in 1%NaCl stresses were lowerthan that in no NaCl stresses (CK), but its salt-tolerant ability of the callus warty were higher than that in no NaCl stresses (CK), its induce percentage of the callus wartyand seedling were doubly increased.3. The system of induce, selection and high propagation coefficient of salt-resistant mutant in kenaf (Hibiscus cannabinus L.) in vitro were established.4. The cultivated technology for the seedling of salt-resistant mutant in kenaf(Hibiscus cannabinus L.) in vitro were established. The seedlings of salt-resistantmutant were planted successfully in field.