| The screened rhizobacteria strains were obtained from microorganism resources and diversity laboratory of Gansu Agricultural University to produce the plant growth-promoting rhizobacteria (PGPR) bio-fertilizers. The PGPR bio-fertilizers were applied on oat (Avena sativa) in the alpine pastoral region to a field experiment, then the growth index and quality characteristics were studied. The results were as follows:1. The quality detection of the bio-fertilizers showed that the quantity of carriers in the solid or liquid nitrogen fixating bio-fertilizer, phosphate solubilizing bio-fertilizer and compound bio-fertilizer were over 108/g after storage for 30, 60, 90 or 120 days at room temperature. It would be decreased down to 106/g when stored for 150 days and then it dropped rapidly with contamination. This suggested that the storage expiration of the PGPR bio-fertilizer should not more than 4 months, also, inoculums amount could be increased when producing.2. The bio-fertilizers could greatly promote the growth of oat. Under the application of half amount chemical fertilizer and half amount PGPR bio-fertilizer in solid form, the height, above ground dryweight, root dryweight, main root length, root volumn and chlorophyll content in different growth stage were significantly higher than contrast. It was no significant difference compared with the treatment used chemical fertilizer only and its effect better than the treatment applied bio-fertilizer only.3. There was significantly effect to the growth of oat at heading stage under the treatment of half amount chemical fertilizer and half amount PGPR bio-fertilizer in solid form. It showed that the height were increased 14.14%～62.31% compared with only chemical fertilizer treatment, and above ground dryweight enhanced 26.96%～40.71% compared with contrast, up to greatly significant level. Root dryweight were added 24.96%～56.47% and showed no significant difference between the treatment of half amount P chemical fertilizer and half amount phosphate solubilizing bio-fertilizer in solid form and the treatment of compound chemical fertilizer. Main root length and root volumn increased 18.60%～23.27% and 41.83%～47.28% respectively, and they were showed no significant difference compared with the treatment of chemical fertilizer only.4. The crude protein(CP)content of oat was greatly higher than that of contrast but the the content of neutral detergent fiber (NDF) and acid detergent fiber (ADF) were significantly lower than control under the application of PGPR bio-fertilizer only or half, especially the use of half amount P chemical fertilizer and half amount phosphate solubilizing bio-fertilizer in solid form. The crude fat (EE) content was greatly improved under the use of PGPR bio-fertilizer, especially the control of half amount phosphate solubilizing bio-fertilizer or half amount compound bio-fertilizer included.5. There were significantly effect to the content of crude protein and crude fat at jointing stage under the control of PGPR bio-fertilizer only or half. It showed that crude protein content were increased 23.07%～39.89% compare with the contrast and the treatmeant of 1/2N was greatly significant compared with the others. The crude fat content were added 2.55%～18.88%, and those control of 1/2N,1/2F和P even showed great significant difference compared with the treatment applied chemical fertilizer only.6. The content of neutral detergent fiber (NDF) and acid detergent fiber (ADF) could be significantly decreased at heading stage and dough stage under the use of PGPR bio-fertilizer only or half. At heading stage, NDF content (except the control of N) were reduced 3.93%～8.67%, especially the treatment used half amount chemical fertilizer and half amount PGPR bio-fertilizer. The ADF content were decreased 4.60%～8.48% compared with the contrast under the control applied bio-fertilizer as solid form only, especially the application of phosphate solubilizing bio-fertilizer only or half showed significant difference compared with that of chemical fertilizer only. At dough stage, the NDF content were reduced 4.90%～7.46% under the treatment of 1/2F,1/2P,P and N, and showed greatly significant difference compared with that applied chemical fertilizer only. The ADF content were decreased 1.05%～8.75% and showed significant difference compared with the contrast under the control of half amount chemical fertilizer and half amount PGPR bio-fertilize.7. The P content of Oat was influenced to some extent by the use of PGPR bio-fertilizer. It could significantly increase the P content of Oat root at different growth stages. The P content of Oat at jointing stage was greatly improved under the trearment of half amount phosphate solubilizing bio-fertilizer or half amount compound bio-fertilizer included. Here the P content of the above ground part were increased 6.94% and 3.71% respectively, compared with the contrast, and the P content of root were also added 35.64% and 19.00% respectively. 8. The effect of compound bio-fertilizer in liquid form was significantly no better than that of bio-fertilizer in solid form. Then further study could be carried out to find out. |
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