Effects Of Different Energy Intake On The Expression Of Fatty Acid Synthase Gene And Obese Gene In Sheep

Twenty four adult female Small tailed Han Sheep were randomly allocated into three groups fed with different energy level diet (standard energy, low energy and high energy) respectively. The expression level of fatty acid synthase (FAS) mRNA and obese (ob) mRNA in the ventral subcutaneous adipose tissue, mesentery adipose tissue and tailhead adipose tissue of sheep were quantified by semi quantitative RT-PCR. We also studied the expression level of FAS and ob mRNA in the tailhead adipose tissue of sheep fed with different energy level diet (standard energy, low energy and high energy). Meanwhile we analysed the correlation among the expression of FAS mRNA, ob mRNA and body weight. The result showed:1. The expression of FAS mRNA in the ventral subcutaneous adipose tissue were significantly different compared with tailhead adipose tissue and mesentery adipose tissue (P<0.01),but there was no significant difference between tailhead adipose tissue and mesentery adipose tissue(P>0.05).2. The expression of ob mRNA in tailhead adipose tissue were significantly different compared with ventral subcutaneous adipose tissue and mesentery adipose tissue (P<0.01),and the expression of ob mRNA in mesentery adipose tissue were significantly different compared with ventral subcutaneous adipose tissue (P<0.01).3. The expression level of FAS mRNA in the tail adipose tissue of Small tailed Han Sheep fed with high energy diet was the highest, and significant higher than those fed with standard energy diet (P<0.01). The expression level of FAS mRNA in the sheep fed with standard energy diet was significant higher than those fed with low energy diet (P<0.01).4. The expression level of ob mRNA in the tail adipose tissue of Small tailed Han Sheep fed with high energy diet was the highest, and significant higher than those fed with standard energy diet (P<0.01). The expression level of ob mRNA in the sheep fed with standard energy diet was significant higher than those fed with low energy diet (P<0.01). 5. The expression level of FAS mRNA correlated positively with body weight (R= 0.954, P<0.01); the expression level of ob mRNA correlated positively with body weight (R=0.953, P<0.01); There was a positive relationship between the expression level of FAS mRNA and the expression level of ob mRNA...