Development And Identification Of Triticum Asetivum-Haynaldia Villosa Ditelosomic Addition Lines And Translocation Lines Involving Chromosome 2V And Application Of Fluorescence In Situ Hybridization

Five ditelosomic addition lines of 2VS, three ditelosomic addition lines of 2VL, twowheat-Haynaldia villosa translocation lines 2VL/W and 2VS/W were identified from theF₂ and F₅ progenies of the high pairing material and wheat-Haynaldia villosasubstitution line 2V(2D) by mitotic and meiotic analysis combining with chromosomeC-banding and fluorescence in situ hybridization (FISH). Additionally, cytological andphenotype results indicated that the gene controlling bristles on glume ridges was locatedon the short arm of 2V. A monoclone TAC-11 selected from the TAC library of wheat-Haynaldia villosa translocation linewas used as probe in FISH technique to study the distribution of TAC-11 in Triticum aestivum, Hordeumvulgare, Secale cereale, Haynaldia villosa, Roegneria kamoji, T. durum-Haynaldia villosa amphiploid,T.aestivum-Thinopyrum bessarabicum amphiploid and T.aestivum-R.kamoji addition line. The resultsindicated that TAC-11 was not a species-specific repetitive sequence. It distributes on the allchromosomes of Triticum aestivum cv. 'Chinese Spring', Hordeum vulgare, Secale cereale andRoegneria kamoji and showed strong signals. The signals on the chromosomes of Thinopyrumbessarabicum was weaker than that of Triticum aestivum. Very weak signals were observed on allchromosomes of Haynaldia villosa and no signal was observed on the chromosome Lr.14 of Leymusracemosus, indicating rarely contribution of TAC-11 exists in Haynaldia villosa and Leymus racemosus.In this study, we found all the the secondary constriction regions of the satellite chromosomes ofT.monococcum, T.dicoccoides, Triticum aestivum, Hordeum valgare, Haynaldia villosa, Avena sativaand Roegneria kamoji have 45S rDNA sites by successive chromosome C-banding and FISH technique.Some non-satellite chromosomes have additional 45S rDNA sites in some species. A pair of loci of 45SrDNA have been located on the terminal region of the short arm of Roegneria kamoji1Rk^#1chromosome according to the 45S rDNA chromosomal distribution in Roegneria kamoji andTriticum aestivum-Roegneria kamoji 1Rk^#1disomic addition line.