The Tissue Culture And ISSR Analysis Of Biological Diversity About Lycoris Sps.

Two specieses, Lycoris radiate and Lycoris aurea, were selected for the studying of artificial propagation in vitro. A few specieses of Lycoris, from the southeast area of China and part of locations in Japan, were chosen to research their karyotype differentiation and to measure their genetic diversity by ISSR analysis. The results could be summarized as follows.1. The vegetative propagation conditions of Lycoris aurea and L. radiate in vitro was studied by two-scaling. Different illuminate condition had an effect on the bulblet formation rate. To the species of L. radiate, the rate was higher in the state of 16h 800-12001x illumination than in the darkness. The medium also affected the new bulblet formating rate. When the culture was MS medium + 0.2 mg/L NAA + 4 mg/L 6-BA, the bulblet formationg rate of L. aurea was 220%;at the same time, when the medium was MS + 0.2 mg/L NAA + 2 mg/L 6-BA, the rate of L. radiate was 108%. Different phytohormone had different influence on the rooting of the two species. NAA is better for rooting than IBA. From the point of organic addition, diatomite remarkably improved the bulblet formation rate of L. aurea, but active carbon restrained the rate. When sucrose concentration reached 6% the weight of bulblet of L. radiate could be the heaviest. MS +4mg/L 6-BA +0.5mg/L NAA was optimal for the bulblet multiplication and the "—" shape excision of bulblet could'obtain more bulblets than "＋" shape excision.2. The chromosome number of all the studied triploid Lycoris, i.e. Lycoris radiate was found to be 2n=33, but it appeared different genetic structure when it was located in different area. The karyotype formula of the material from the city of HECHUAN in CHONGQING province was 2n=33=3T+30t, while the karyotype formula of...