Constructing Of Genetic Linkage Map And QTL Mapping Of Important Agronomic Traits In Two Soybean Populations

China was the original place of soybean and contained abundant germplasm resource, digging choiceness gene in germplasm resource to improve our soybean breeding level were necessary. In this study, a northeast spring soybean F₂ population derived from Suinong 14×Suinong 20 containing 580 plants was constructed, and 94 plants were randomly selected for constructing genetic linkage map and QTL analysis for yield-related traits, seed quality traits and other important agronomic traits in F₂ and F₄ population. At the same time, based on genetic linkage group map constructed using F₂ population derived from Huanghuai summer soybean Kexin 3×Zhonghuang 20, QTL analysis for important agronomic traits in F₃ and F₄ generations was also performed. Comparation of genetic linkage groups and QTL mapping in different populations. addition the density of genetic linkage group map to dig repeatable QTLs of important agronomic traits and establish the basic for marker aided selection.Main results were as follows:1. Constructing genetic linkage group map using F₂ population derived from Suinong14×Suinong20.A total of 172 SSR(Simple Sequence Repeat)markers showing polymorphic between Suinong14 and Suinong20 were used to construct genetic linkage group map. This genetic map consisted of 27 linkage groups, included 120 SSR markers, and covered approximately 1581.6 cM of soybean genome. The average of distance between markers was 13.18 cM. The number of SSR makers on each linkage group varied from 2 to 11. The length of each linkage group ranged from 9.2 cM to 206.6 cM. A total of 23 linkage groups was included in the common linkage map which were constructed by Cregan et al.2. Mapping a set of QTLs about important agronomic traits in different populations and generations.(1)Using F₂ population derived from from Suinong 14×Suinong 20, a total of 11 QTLs associated with 7 traits (plant height, number of nodes, number of 1-seed pods, number of 4-seed, biomass, 100-seed weight and oil content)were detected, and they distributed on seven linkage groups, and the R2 were between 5.55%-33.06%. Of 11 QTLs, four QTLs were in the same or similar location as those QTLs previously reported. In this study, QTL cluster was also found. For...