Pathogen Identification Of Bacterial Diseases On Five Cultural Plants And Bacteriocides Screening Indoor

Five new bacterial diseases of Ligularia fischeri, Vigna angularis , Brassica juncea var.japonica, Aconitum coreanum and Silybum marianum in Jilin province were firstly reported in the world; and their sympotoms of the diseases were described in detail. According to the configuration of the pathogens observed by electrical microscope, Gram staination, pathogenicity, cultural characters, physiological and biochemical characters, as well as G+C mol%, the pathogens were identified respectively . Bacteriocides for the pathogens were screened indoor.The main symptoms of the bacterial disease on Ligularia Jischeri were rot. The strains were straight or curved rods; polar flagellum one; Gram-negative; could infect Ligularia fischeri and Brassica pekinensis; 41℃ growth negative ; oxidase positive; potato rot positive; arginine dihydrolase positive; levan positive; tobacco hypersensitivity positive; gelatin liquefaction positive; nitrate to N₂ positive; nitrate reduction positive; used glucose, sucrose, trehalose, sorbite for growth and didn't use tartrate and β-alanine for growth; rochelle salt negative; carrot rot negative; G+C mol% -66.3%.According to parts of 《Bergey's Manual of Determinative Bacteriology》 9th ed,it confirmed that the disease was caused by Pseudomonas marginalis, and the characters were similar to P.marginalis pv.pastinacae, but couldn't infect Pastinaca sativa .So it was a new pathovar of Pseudomonas marginalis ,named as Pseudomonas marginalis pv. ligularia pv. nov..The main symptoms of the bacterial disease on Vigna angularis were canker of stalk.The strains were straight or curved rods; polar flagellum one; Gram-negative; could infect Vigna angularis, Glycine max, Vigna radata, Vigna umbellate, Phaseolus vulgaris, Pisum sativum, Pisum sativum var.humlie; oxidase negative; nitrate reduction negative; 5% glucose agar mucilage positive; esculin hydrolysis positive; starch negative; milk hydrolysis positive; H₂S positive; acid from L-arabinose, D-mannose, D-galactose, cellobiose, trehalose and fructose; 5%NaCl positive; G+Cmol%-69%. According to parts of 《Bergey's Manual of Determinative Bacteriology》 9th ed, it confirmed that the disease was caused by Xanthomonas campestris pv.alfalfae (Riker, Jones et Davis 1935 )Dye 1978. The main symptoms of the bacterial disease on Brassica juncea var.japinica and Silybum marianum were soft rot.The strains of two diseases were straight or curved rods;...