The Research On Tissue Culture Of Paeonia Suffruticosa And Paeonia Lactiflora Pall

This test was based on Paeonia suffruticosa and Paeonia lactiflora Pall for materials. Paeonia suffruticosa was mainly studied on embryo culture, shoot culture and factors that affected the vitrification, and Paeonia lactiflora Pall was studied on dormant shoot culture, callus induction and prevention of browning. The results were as follows:1. Embryo culture of peonyBy using Feng Dan mature embryos as explants, a procedure for rapid propagation of mature embryo culture was established. The results of experiments showed that the effect of different media on peony bud induction was significant, MS can be used as Feng Dan basic embryo culture medium, the germination rate of Feng Dan embryos in medium MS+6-BA 1.0 mg/L+GA3 1.0 mg/L was best, the medium MS+6-BA 1.0mg/L+KT 1.0mg/L was suitable for subculture medium, the rooting rate of seedlings in the medium 1/2MS+IBA 2.0 mg/L was up to 43.2%,the induction of germination rate increased significantly after 45d of cold treatment.2. Dormant bud culture of peonyBy using dormant buds of Paeonia suffruticosa as explants, Disinfection with 0.1% HgCl2 10min was better to reduce pollution and vitrification. The effect of different concentrations of 6-BA on germination rate of dormant buds of different varieties was different. Orthogonal design of the Luo Yang Hong in vitro multiplication was studied, the results showed that 6-BA and KT were important growth regulators of Luo Yang Hong in vitro multiplication, the optimal combination of Luo Yang Hong in vitro multiplication was MS+6-BA 1.0 mg/L+KT 1.0 mg/L, multiplication coefficient was 2.15.The multiplication coefficient of different varieties on same medium was significantly different. Luo Yang Hong plantlets rooted well in the medium 1/2MS+ IBA 1.0 mg/L+sucrose 30g/L + agar 6g/L, rooting rate can reach 82.5%, rooting rate can be improved by two steps rooting method. 3. Prevention of vitrification of peony plantletMS medium was chosen as the basic medium and the main factors such as cultivars, agar, 6-BA, macroelement, sucrose and intensity of illumination were investigated on vitrification of plantlets from Paeonia suffruticosa. The results showed that different cultivars of Paeonia suffruticosa in tissue culture process appeared vitrification in degree. Increasing the concentration of agar in culture medium, reducing the concentration of 6-BA, reducing the concentration of macroelement and the increasing intensity of illumination or using the natural light were possible to reduce the vitrification of plantlets effectively.4. Dormant bud culture of herbaceous peonyBy using dormant buds of Paeonia lactiflora Pall, acquisition time of dormant bud affected the germination rate to certain extent; February was the best time of the herbaceous peony dormant buds acquisition. Effects of different medium on germination rate of dormant buds of different varieties were significantly different; 1/2MS was quite suitable basic culture medium for induction of germination herbaceous peony dormant bud. the optimal combination of Zhong Sheng Fen in vitro multiplication was MS+6-BA 1.0 mg/L+KT 0.5 mg/L,multiplication coefficient was 2.39.Different concentrations of IBA affected the rooting rate, root length, root number, the rooting of herbaceous peony plantlet was the best in medium 1/2MS+IBA 2.0 mg/L, rooting rate can reach 58.0%.5. Callus Induction and browning inhibition of herbaceous peonyThis test used Paeonia lactiflora Zhong Sheng Fen as experiment material. Comparing the effect of different explants, basal mediums and combinations of hormones to callus induction, the results indicated: to use plantlet petiole as explants, on the medium MS+6-BA 1.0mg/L+2,4-D 0.5 mg/L+sucrose 30 g/L+agar 6 g/L, induction rate reached to 80.0%.In the research of the effect of basal medium, different concentrations of inhibitor and culture condition to the browning, the result implied browning was slighter in the basal medium 1/2WPM .Anti-browning had remarkable effect to browning prohibited;AgNO3 took on the best browning prohibited effect in all of them, low-temperature and darkness could reduce browning in degree.