| Betula luminifera H. Winkl. is one of the with fast growth broad-leaf and high-quality timber production tree species in China. The adaptability of cultivation is wide and lumbers are used popularly. As an important in southern China's precious timber tree planting promotion.The fiber traits in six natural populations located in Linan, Guizhou, Jiangxi, Fujian, Guangxi and Qingyuan were compared, and lignin gene 4CL was cloned, these results as follow:1. Taken separately of 30 strains which from Zhejiang Qingyuan Jinzifeng, 22 strains from Fujian Youxi , 5 strains which from Linan Taihuyuan, 19 strains which from Jiangxi Jiulianshan, 22 strains which from Guangxi Huaping and 17 plants which from Guizhou Xiuwen, in total 115 strains of Betula luminifera and (1.3 meters) in diameter drilling wood core samples. Nitric acid method with wooden fiber, calibration segregation of measuring the microscope measuring wood fiber length, width, fiber lumen diameter of fiber, and the calculating cellwall-lumen. Reuse SPSS and Excel to the relevant analysis of variance analysis.The fiber length is mostly between 1200μm and 2000μm, and the fiber width between 15μm and 21μm. fiber cellwall-lumen between 05~0.7, The variation and characteristics of the wood properties of Betula luminifera six natural stands within and between the populations were analyse. Result indicates that there are great differences in fiber length and fiber cell wall-lumen between the populations of the Betula luminifera.The frequencies of fiber length and width follow an approximate normal distribution. while an insignificant difference was found in fiber width. Jiangxi average fiber length of 18 reached the maximum 2112μm, and Guizhou the average fiber length of 13, the minimum is only 793μm; average maximum fiber width is 24 Qingyuan 35.59μm, the smallest in Guizhou 8 is 23.33μm. Guangxi 4 cell wall-lumehan the average maximum is 1.047, Guangxi 18 cell wall-lumehan of the smallest average is 0.386.2. Isolation of high-quality RNA from plant tissue is the prerequisite to carry out gene cloning and expression analysis. According to different component composition of different plant population, RNA extraction have different difficulties. In this paper, young leaves of Betula luminifera were used to establish an optimum method for isolating total RNA. Because polysaccharides and polyphenol compounds are rich in, Betula luminifera, the CTAB method were improved by adding PVP andβ-ME to remove polyphenol compounds, adding ethanol to remove polysaccharides and LiCl to precipitate RNA. The bands of the isolated RNA were regular and clear. Using the isolated RNA as template for reverse transcription, Full length of Actin gene was cloned by using RACE method. These results indicated that CTAB-LiCl method could isolate high quality total RNA, which can be used for subsequent molecular biological experiments such as reverse transcription and cDNA library construction.3. We cloned a cDNA encoding 4-coumarate-CoA ligase in 4-coumarate-CoA ligase from Betula luminifera by RT-PCR and 5', 3'RACE methods. Sequence analysis showed it is 1,983bp long and contains a single open reading frame (ORF, 69 ~1,697bp) encoding a protein of 542 amino acids (GeneBank accession number: FJ410448). The protein has a motif containing 12 amino acids and a functional domain which is AMP-Binding domain involving the 4CL enzyme activity. The phyloenetic tree of 4CL showed it has a close relation with Betula platyphylla. The result of Sothern blot implicated Bl4CL have more than two copies in genome of Betula luminifera. Based on the expression level of gene 4CL in leaf, the data from flower was decreased by 0.89, while the expression level of root and stem were increased by 5.04 and 4.59 respectively. |
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