Preliminary Study On Chemical Components And Quality Control Methods Of Medicinal Plant

The Anredera cordifolia(Tenore) van Steen is bulbule of Boussingaultia gracilisMiers var. pseudobaselloides Bailey,which origin is Brazil.Boussingaultia gracilisMiers var. pseudobaselloides Bailey is a new kind of crude drug of homology of medicine and food which was introduced of domestic in recent years.The present study provided the primary methods of extraction and isolation technology,and collcted the stem, leaf and flower of Boussingaultia gracilis Miers var. pseudobaselloides Bailey ,and determined the general flavonoids in different parts of Boussingaultia gracilisMiers var. pseudobaselloides Bailey by ultraviolet spectrophotometry,and determined adenosine in different parts of Boussingaultia gracilis Miers var. pseudobaselloides Bailey(stem,leaf and flower)by HPLC,and Simultaneous determined the content of uracil and desmosflavon in different parts of Boussingaultia gracilis Miers var.Pseudobaselloides Bailey (stem,leaf and flower)by HPLC.The whole plants of Anredera cordifolia(Tenore) van Steen were extracted with 75 %aqueous alcohol to give an alcoholic extract which was extracted successively with the same volume of petroleum benzene, acetic ether and n-butanol.In order to isolate these extracted parts of Anredera cordifolia(Tenore) van Steen,various chromatographic techniques were performed.eight compounds were isolated and identified with UV,IR,NMR and MS data and physical-chemical properties. They were adenosine,desmosflavone, ursolic acid,uracil, octadecanoic acid, oleanolic acid, (3-sitosterol and daucosterol. The compounds adenosine、uracil and octadecanoic acid were isolated from this genus for the first time.The concentration of general flavonoids in different parts of Boussingaultia gracilisMiers var. pseudobaselloides Bailey (bulbule,flower,leafandstem)were determined with colorimetry. Eldirn was standard product. The absorbance of general flavonoids in samples was determined at 499 nm.The calibration curves of eldrin was linear over the range of 2.80-33.7 ug-mL-1 (r=0.999 3,n=6). The mean reeovery were 97.6%(RSD=1.0%).The assay which was founded was simple,fast and accurate to measure the concentration of general flavonoids in Boussingaultia gracilisMiers var. pseudobaselloides Bailey(bulbule,flower,leaf and stem).So the assay can be used as one of the method to control the quality of Boussingaultia gracilisMiers var. pseudobaselloides Bailey.The adenosine in different parts of Boussingaultia gracilisMiers var. pseudobaselloides Bailey(bulbule,flower,leaf and stem) were determined by reversed phase high performance liquid chromatography (RP-HPLC). The determination was performed on a Kromasil C18 column(250 mmx4.6 mm,5μm) with a solvent system consisting of methanol--water (V:V=10:90) was used,the flow rate was 1 mL.min-1,and the detecting wavelength was set at 254 nm. The Linear in the range of 1.86-18.6 mg·L-1 with correlation coefficient of 0.999 2 (n= 6). The average recovery of calycosin was 98.0% with RSD of 1.3%(n= 9). The assay was found to be simple and accurate to measure the concentrations of adenosine in Boussingaultia gracilisMiers var. pseudobaselloides Bailey.The uracil and desmosflavon in different parts of Boussingaultia gracilisMiers var. pseudobaselloides Bailey(bulbule,flower,leaf and stem) were determined by reversed phase high performance liquid chromatography (RP-HPLC). The determination was performed on Kromasil C18 column (250 mmx4.6 mm,5μm) with a solvent system consisting of acetonitrile(A)-0.05%H3PO4(B):3%A (0-6 min);3%A-60%A (6-8 min);60%A-80%A (8-13 min);80%A (13-20 min) was used,the flow rate was 1 mL-min-1.and the detecting wavelength was set at 254 nm, the column temperature was 35 temperature. There was good liner relationship between the peak areas and the sample contents injected at the ranges of 0.705-7.05μg·mL-1 r=0.999 9 (n=6) and 0.729-7.29μg·mL-1 r=0.999 9 (n=6) for uracil and desmosflavon respectively. The average recovery rates of uracil and desmosflavon were 99.2%(RSD=1.3%) and 99.0 %(RSD=1.2%),respectively. The method is sensitive,accurate,rapid,and it is suitable for the quality control of Boussingaultia gracilis Miers var.Pseudobaselloides Bailey.