| Background:Pulmonary fibrosis (pulmonary fibrosis, PF) is between serious lung caused by a variety of quality chronic disease. The main pathological features of its occurrence early acute lower respiratory tract inflammation based, including alveolitis, interstitial pneumonia, alveolar epithelial damage, macrophages, neutrophils and other inflammatory cell infiltration, along with a large number of fibroblasts cells and myofibroblast proliferation of pathological transition, leading to post-extracellular matrix (extracellular matrixReal-time, ECM) components within the alveolar and interstitial deposition of fibrous tissue disorder caused by excessive lung tissue repair structures replace normal lung tissue structure, The essence of alveolar dajnage, collagen deposition, eventually leading to pulmonary fibrosis. In recent years, with further research on the pathogenesis of pulmonary fibrosis, and this team in the long-term clinical practice, according to the clinical experience of chemical fiber series party was founded, its water decoction of pulmonary fibrosis patients have a certain effect. In this paper, we focus on "Qi and Yin Deficiency and blood stasis" Huaxianfang critical path of EMT genes E-cadherin (E-cadherin, E-cad), a-smooth muscle protein (a-smooth muscle actin, a-SMA), the impact vimentin (vimentin) expression, and to explore the impact of the Recipe on bleomycin pulmonary fibrosis in rats at the molecular level, as well as the animal model for the effectiveness of the two parties at the time of treatment.Objective:By observing bleomycin rat model of pulmonary fibrosis in classical Chinese medicine treatment in E-cadherin, a-smooth muscle protein, vimentin expression changes at the molecular level to explore, "Qi and Yin Deficiency and blood stasis "a series of chemical fiber and chemical fiber Fang Fang (HA), fiber B side (HB) impact on bleomycin pulmonary fibrosis in rats.Methods:3-month-old SPF level 72 healthy SD rats, weighing 180 ± 20g, male and female. Randomly divided into control group and model group and modeling, modeling survival after rats were to begin treatment, continuous 28d. Observe the animals in general, to modeling,7,11,23,28 day, were selected in the model group rats corresponding time points; the remaining 28 days of treatment in each group sampled. Weighing, femoral artery blood, lung apparent change was observed. Left lung group neutral formalin fixed 10%, HE, Masson staining underwent alveolitis and pulmonary fibrosis grading grading. Lung tissue mRNA mRNA expression for performing QPCR detect E-cadherin, a-SMA, vimentin mRNA’s. Spss19.0 data using statistical analysis.Results:Pulmonary index:B28 lung coefficient significantly greater than P, HA, HB group (P<0.01). Pathological observation:after modeling in the lung tissue of rats on the 7th day full of inflammatory cells in the alveolar interstitial lung, interstitial lung widened. The first 11 days of inflammatory cells decreased but accompanied by bleeding spots appeared, among the first 23 days of pulmonary interstitial inflammation relief, reduce bleeding points scattered, accompanied by collagen deposition. The first 28 days shows a large number of collagen deposition, the initial pulmonary fibrosis alveolitis mainly based, mainly late in fibrosis. Associated gene expression:B, P, HA, HB gene expression in lung tissue of rats in the group E-cad down, and mRNA expression of mean and HB Group E-cad were higher than the model group and statistically significant (P<0.05), mRNA expression of HA and HB group E-cad group were significantly different (P<0.05). B28, mRNA expression of a-SAM emergence of HB significant difference (P<0.05), mRNA expression of HA and HB of a-SAM includes significant difference (P<0.01). Increase the amount of mRNA expression of HB’s vimentinm significantly lower than B28 (P<0.05), mRNA expression of a-SAM’s HA and HB are significant differences (P<0.05), and the mRNA expression of HB vimentinm below HA.Conclusion:1 fiber A Party (HA) and fiber B side (HB) bleomycin-induced pulmonary fibrosis alveolitis and pulmonary fibrosis have a certain effect. Fiber B side effect of pulmonary fibrosis may be related to a-SMA, vimentin, E-cad expression changes related to the development process that is delaying the EMT.2 bleomycin-induced pulmonary fibrosis in animal models for studying classical chemical fiber series Party of pulmonary fibrosis, "Activating Blood circulation", the results show fiber B side is more suitable for the disease, disease. |
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