A Preliminary Study On Proteomics Of Psoriasis Vulgaris (Blood Deficiency Syndrome) Based On ITRAQ Technique

Objective:To preliminary and study on the blood deficiency psoriasis vulgaris with serum protein,in order to find the difference proteome of blood deficiency psoriasis vulgaris compared with normal.And quantitative analysis of its proteome differences, thus providing a basis of microscopic quantitative for syndrome Differentiation in blood deficiency psoriasis vulgaris. While providing a method and objective, stable indicators for the clinical treatment and clinical evaluation of psoriasis vulgaris, and provide ideas for microscopic quantitative research syndrome differentiation to other types of psoriasis vulgaris or other disease.Methods:Using iTRAQ technology, To quantitative and study on the blood deficiency psoriasis vulgaris with serum protein, and carried statistic analysis on difference proteome.Results:1, Blood deficiency psoriasis vulgaris serum protein group and the normal group does exist obvious differences proteome.2,This experiment were quantified 208 proteins identified (Protein), blood deficiency in patients with psoriasis vulgaris serum proteome comparison with normal human serum proteome, which has 109 proteins there were significant differences(P<0.05), the protein has significant differences have 26.3, The differences is mainly concentrated protein expression of several proteins in order to look at the group:coagulation-related proteins、 lipoprotein-protein、immune-related proteins、tumor-associated proteins、 cytokeratin related protein、hemoglobin protein group、class of extracellular matrix proteins、vitamin-related protein and so on. Conclusion:Blood deficiency psoriasis vulgaris serum protein group and the normal group does exist obvious differences proteome. These differences in the protein group, coagulation-related proteins、 lipoprotein-protein、immune-related proteins、tumor-associated proteins、 cytokeratin related protein、hemoglobin protein group、class of extracellular matrix proteins、vitamin-related protein and so on. maybe as objective indicators of blood deficiency psoriasis vulgaris and non-blood deficiency psoriasis vulgaris.