Effects Of Down - Regulation Of MAEL And Up - Regulation Of CDH1 Gene On The Migration And Proliferation Of HepG2 Cells

Objective: To investigate the migration, invasion and proliferation of Hep G2 cell when up-regulating CDH1 gene and down-regulating MAEL gene.Methods: The si RNA of targeting MAEL and the saRNA of targeting CDH1 promoter were transfected into Hep G2 cells with transfetion reagent. According to the different of transfection mixture cells were divided into five groups:CT group 、 NC group 、 778groups、215 groups and 778+215 groups. q PCR was used to detect the expression of MAEL and CDH1, Western Blot was used to detect the expression of MAEL, E- cadherin,Caspase-3 and Bcl-2. MTT assay was used to detect the cell proliferation, scratch assay was used to detect the cell migration, the Transwell invasion assay was used to detect the cell invasion.Results: si RNA-778 and saRAN-215 was used to treat HepG2 cells.The qPCR showed that the si RNA-778 significantly reduced the expression of MAEL gene(p<0.05) and the sa RNA-215 was significantly upregulated CDH1 gene(p<0.05). The Western blot shows that the expression of MAEL and Bcl-2 were significantly reduced(p<0.05) and the expression of E-cadherin and Caspas-3 were significantly increased(p<0.05). Both the cell proliferation and the scratch healing were significantly lower(p<0.05), the number of Hep G2 cells that passed through the basement membrane was significantly reduced(p<0.05) than CT group, and CT group and NC group has no Statistical significance(p>0.05). The proliferation, the migration and the invasion of the co-transfected cell were better inhibited than the si RNA-778 or sa RAN-215 transfected cell(p<0.05).Conclusion: The expression of MAEL genes can be effectively downregulated by si RNA-778, and the expression of CDH1 gene can be effectively upregulated by sa RNA-215. The migration, invasion and proliferation of Hep G2 cell were reduced. All these phenomena may related with the suppression of epithelial-mesenchymal transformation. And it may enhance apoptosis via Caspase-3/Bcl-2 signaling pathway.The regulation effect of si RNA-778 and sa RNA-215 combination is superior to a single gene.