Study On The Mechanism Of Human Umbilical Cord Mesenchymal Stem Cell Microcapsules On Invasion And Metastasis Of Bladder Tumor

Objective: We recently reported the anti-cancer effect of microvesicles(MVs) derived from human Wharton’s jelly mesenchymal stem cells(h WJMSCs) in the inhibition of bladder tumor cell growth in vitro and in vivo. Methods: In the present study, we investigated the anticancer effects of MVs on the migration and invasion of human T24 cells. MVs inhibited the cell migration and invasion of T24 cells by the wound healing and transwell assays, respectively. Mechanistically, MVs reduced the invasiveness capability of bladder cancer cells in a manner associated with decreased activity of MMP-2 and MMP-9. Further, MVs treatment suppressed features of epithelial-to-mesenchymal transition(EMT), a process crucial for metastasis.Results: Western blot analysis showed that the proteins of slug and p-AKT were reduced in MVs-treated cells. However, the reversion of EMT affected by MVs was attenuated by an activator of PI3K/AKT, insulin-like growth factor 1(IGF-1). Therefore, MVs inhibiting migration and invasion behaviors of T24 cells might be mediated through blocking PI3K/AKT/slug signaling pathway, resulting in the reversion EMT. Conclusion: These results indicate that MVs can reverse the EMT of T24 cells. The possible underlying molecular mechanisms are through inhibition of the PI3K/AKT/slug signaling pathway.