| Aim: to explore the changes in the density of blood vessels after anterior cruciate ligament(ACL) injure in rabbit and provide theoretical basis for ACL reconstruction with preservation of remnant.Methods: 30 healthy New Zealand White rabbits(F/M) were divided into5 groups randomly, each group had 6 rabbits. Except the control group,the right hind legs of rabbits in other four groups were all cut off. The rabbits in the four experimental groups were killed at 2, 4, 8 and 12 weeks respectively, and the ACL remnants were collected. The ACL specimens were dyed by HE staining method and immune histochemistry method to observe histological features and vessel density.Result: Compared with control group, the vessel density of ACL was higher at 2 weeks, reached peak at 4 weeks(P<0.05) and gradually decreased at 8 and 12 weeks. The vessel density at 12 weeks was lower than control group(P<0.05).Conclusion: Vessel density of the ACL remnants had changed over time after ACL injure-first increased and then declined. The change of vessel density implied early ACL reconstruction with preservation of remnant was beneficial for the graft revascularization. |
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