Mouse Uterine Decidual Stromal Cells Promote The Proliferation Of Dendritic Cells

Objective: The immune system is a precise reaction system. The initiation,progress and calm down of the immune response is precisely regulated by the immune system. Studies on the regulatory mechanisms of the immune response are important, and it can not be ignored that the regulation of the immune microenvironment on the immune response. Immunocyte are the principal components of the immune response, and dendritc cells( DC) are especially important and lies in the centre of the immune response. Study on influence of the immune miroenvironment on the biological functions of DC is very important. The pregancy is a complicated physiological process,and the maternal immune system do not reject the allogeneic fetus. It means the maternal immune system established the immune tolerance to the fetus.The establishment and maintenance of the maternal-fetal tolerance is a dynamic balance involved by multipal complicated mechanisms. We established the mice decidual stroma cells and bone marrow derived dendritic cells cocultured system, and investigated the effects of decidual stroma cells(DSC)on the proliferation of dendritic cells.Methods: Cells isolated from the mice bone marrow were cultured with GM-CSF and IL-4 cytokines,then cell surface markers and cell proliferation were detected by FACS,and cytokines were assayed by ELISA.Moreover,we developed a co-culture system with DSC and DC,and dynamically observed the effects of DSC on the proliferation of DC.Results: Bone marrow derived DC express high levels of CD11 c, CD80,CD86, MHC-II, and MHC-I molecules. The secretion of IL-12(p70)、IL-6、TNF-a and IL-1b were increased significantly treated by LPS(p<0.05), and the ability to stimulate antigen specific T cell proliferation was also enhanced(p<0.05). In addition, the proliferation of DC was increased after we cocultrued DSC and DC for 10 days.Conclusion: We have successfully developed a co-culture system of mouse uterine DSC and mouse bone marrow-derived DC. We validated that DC could promote the proliferation of antigen-specific T cells and we discussed the molecular mechanisms of interactions between mouse uterine DSC and the DC. We have found uterine DSC of mice have the ability of promoting the proliferation of DC. In this study, we provide experimental evidence of the role of DC in maternal-fetal immunotolerance, which has important theoretical significance and prospects of clinical applications.