Experimental Study On Effect Of Buzhong Yiqi Decoction On Fas / FasL Protein Expression In Spleen Tissue Of A549 Tumor Bearing Mice

Purpose:To provide experimental basis for the treatment of advanced lung cancer and relieve the side effects of chemotherapy by using Buzhong Yiqi Decoction which can“reinforcing earth to strengthen metal”, this paper investigate the effect of the decoction on the protein expression of Fas/Fas L of spleen tissue in A549 tumor bearing mice in vivo.Material and methods:Using the randomized controlled method to divide the 36 SPF clean grade mice into 6 groups:normal group(NM,0.9% sodium chloride solution,1m L),blank control group(BC,0.9% sodium chloride solution,1m L),the high level dose of Buzhong Yiqi Decoction group(BH,0.3276g/m L,1m L),the middle level dose of Buzhong Yiqi Decoction group(BM,0.1638g/m L,1m L),the low level dose of Buzhong Yiqi Decoction group(BL,0.0819g/m L,1m L),DDP group(DG,0.25mg/m L,0.4m L).The tumor-bearing model was built by subcutaneous injection except the normal group.The mice of DG were given DDP every day by intraperitoneal injection after successfully transplanting.The normal saline and different level does of Buzhong Yiqi Decoction were given into other mice by gavage twice a day,and materials were drawn in the tenth day.The expression of Fas and Fas L in spleen tissue of mice were detected respectively by immunohistochemistry,the m RNA level of Fas and Fas L in lymphocyte of spleen tissue were detected respectively by RT-PCR.The experimental data was expressed as the mean ± SEM.The difference among means were examined with ANOVA followed by post-hoc test using SPSS19.0 software.A p value less than 0.05 was considered as statistical significance.Results:1.The average length of spleen of lung cancer mice in BC was 2.17±0.05 cm,which was bigger than NM.The difference was considered as statistical significance(P<0.01),while there was no statistical difference among BH,BM and DG compared with NM. 2.The average optical density of Fas tested by IHC in the spleen tissue of BC was0.231±0.007,which was higher than NM,the difference was considered as statistical significance(P<0.01);The average optical density of Fas L tested by IHC in the spleen tissue of BC was 0.243±0.032,which was lower than NM,the difference was considered as statistical significance(P<0.01).Compared with the β-actin,the relative expression values of Fas m RNA was 83.43±7.329,which was higher than NM,and the difference was considered as statistical significance(P<0.01);the relative expression value of Fas L m RNA was 43.58±6.403,which was lower than NM,and the difference was considered as statistical significance(P<0.01). 3.Compared with BC,the expression of Fas tested by immunohistochemistry and RT-PCR in lymphocyte of spleen tissue of mice in BM decreased significantly,the difference was considered as statistical significance(P<0.01)which had no statistical significance compared with NM.The expression of Fas L in spleen tissue of mice in BM rose obviously,the difference was considered as statistical significance(P<0.01),while difference between BM and NM was considered as statistical significance(P<0.05). 4.There was no statistical difference between the expression of Fas L in spleen tissue of mice in BC and BL detected by immunohistochemistry,while difference was consider as statistical significance between them by RT-PCR.Conclusions:1.The expression of Fas increases and the expression of Fas L decreased in spleen of lung cancer mice. 2.The Buzhong Yiqi Decoction could down-regulation the expression of Fas and up-regulation the expression of Fas L in spleen tissue of tumor bearing mice,which may improve immune function.