| Stroke refers suddenly collapsed, unconscious, hemiplegia, Kouyanwaixie, language disadvantage as the main clinical features of syndromes, stroke clinically into the meridians and organs in two categories. Due to sudden illness, rapid outbreak, clinical performances different, and varied, also known as the "stroke".According to the clinical performance characteristics of stroke, the equivalent of Western medicine cerebrovascular disease, including ischemic stroke and hemorrhagic stroke.After studying at the clinic with the tutor, summed particularly recognized Zhao experience clinical diagnosis of stroke therapy and pathogenesis of the disease identified. Zhao in long-term clinical practice, according to the theory of phlegm and doctrine, think the pathogenesis of stroke for phlegm, premise happens is that the body is deficiency decline, the incidence can be divided into three stages, are the meridians, the organs, stroke sequelae. Therefore, based on the blood disease differentiation Phlegm, clinical with "chronic illness into the network, phlegm stasis closed knot" Outline for the syndrome, and emphasizes that the key blood running smoothly, accumulation of phlegm and blood stasis in the body, can make blood runs sluggish, will produce a variety of diseases. Treatment in acute then treat the acute phase focuses on promoting blood phlegm resuscitation; Slow then treat this, focusing Huoxuetongluo prevent recurrence.Zhao believes Western brain hemorrhage is a Chinese "hemorrhagic stroke" category, the main mechanism of pathogenesis of organ dysfunction, air-lift disorders, phlegm blocking brain awakened, made for stroke. Ditan Tongyu its main treatment method.The purpose of this experiment was to explore the level of protein expression in acute cerebral hemorrhage model under stress changes induced liver cell apoptosis and liver tissue of rats IRAK-2, while the protective effects of Ditan Tongyu Decoction to liver damage.Method:Healthy male SD rats randomly divided into Di Tan Tong Yu group, Cerebral Hemorrhage group, Normal group. The acute hematencephalon model was established by injecting VII collagenase on brain, and serum liver function level, liver tissue pathology were observed in three time point, and hepatocyte apoptosis index was detected by Tunel. And Each group of rat liver tissue IRAK-2 protein levels was detected by using Western Blot, changes of TNF-αccontent by using ELISA. Result:Experiment one:There were more apoptotic cells in Cerebral Hemorrhage group, which had significant differences compared with Di Tan Tong Yu group and Normal group (P<0.05 or 0.01). Experiment two: Rat model of acute cerebral hemorrhage IRAK-2 protein levels,24h cerebral hemorrhage model group than in the normal group was significantly lower, but 48h, with point significantly higher (P<0.01) at 72h,48h, the point where the relative expression levels peaked at significantly lower than the expression level of the Medicines ICH model group of IRAK-2 (P<0.01). Meanwhile, cerebral hemorrhage in the liver was significantly increased TNF-a levels than medicine group and the normal group, the treatment group was significantly lower than that of cerebral hemorrhage. Each group at each time point in rat liver tissue IRAK-2 protein expression and TNF-acontent in a positive correlation. Conclusion: Experiment one:Di Tan Tong Yu decoction can ameliorate the stress-induced hepatic injury in rats with Acute Cerebral Hemorrhage, and has protective effect on hepatocyte apoptosis. Experiment two:IRAK-2 can activate NF-kB activation and induce inflammatory cytokines such as TNF-arelease, leading to liver damage. The characteristics of traditional Chinese medicine has a role in many ways, and can play through different target anti-inflammatory effects, inhibit NF-kB activation, thereby antagonizing TNF-ainhibit the inflammatory cascade of cerebral hemorrhage after liver tissue protective effects. |
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