| Background:Hypertrophic scars conventional treatments, including surgery, steroidhormones, anti-metabolite drugs, immunosuppressive agents and radiation therapy, mostlikely repeat attack or a more serious reaction which limits its use in clinical areas. Inrecent years, application of calcium channel blockers treatment of hypertrophic scars mademore good progress, but little adverse reactions. In this experiment, in order to testtrifluoperazine, in order to test whether trifluoperazine treatment of hypertrophic scars canbe a drug of choice.Purpose:Based on rabbit ear hypertrophic scar model, develop appropriate methodsof administration, dosage, and then through tissue staining, electron microscopy andrelated testing methods, research trifluoperazine on rabbit ear hypertrophic scars, and theimpact of related factors and scar formation, and thus on the basis of certain theories andexperiments, to explain TFP is able to become the treatment of hypertrophic scars optionaldrugs.Method:Choose adult, healthy rabbit24, requiring ear abdominal trauma, noabnormal plaque, feeding observed one week, no exceptions, such as referring to thereference Morris, Li Hui-yuan, and the establishment of hypertrophic scar of rabbit earsGuiying rabbit model approach ear scar model. In order to improve the success rate ofpreparation for surgery scar model, strict aseptic procedures, to operate. Every single sixear wound preparation carried a total of288wounds. Until30days after the rabbit ear scarformation, were randomly divided into three groups, trifluoperazine as a test drug group,triamcinolone acetonide as a positive control group, saline as a control group. Groupingseveral times in the bottom of the base and scar lesions local injection. Amount injected50μg/wound injection, once every three days for six times. Trifluoperazine on dilutionand triamcinolone acetonide should be the same, in order to ensure the unity of theexperimental variables is1μg/μl. After the scar tissue to picric acid magenta (VG)hematoxylin-eosin (HE) staining of scar tissue, scar tissue was observed in collagenfibers, fibroblasts into a number of forms. Because of PCNA and TGF-β1-related factorsand scar formation, so the scar tissue by ELISA and TGF-β1levels of PCNA were measured. For auxiliary prove trifluoperazine hypertrophic scar of rabbit ears feasibilitytherapeutic effect.Result:1.HE staining showed trifluoperazine triamcinolone group composed ofrelatively few fibroblasts, whereas fibroblasts blank relatively large group trifluoperazinequantity of fiber cells below the level of the control group, and triamcinolone acetonide nosignificant difference in the German group;2.VG staining showed trifluoperazine group and triamcinolone acetonide group collagenfibers are relatively sparse and tidy, while the control group was relatively dense collagenfibers disorders, trifluoperazine neat group than the control group, with no significanttriamcinolone group differences.3ELISA assay showed that trifluoperazine inhibits TGF-β1and PCNA effect.Conclusion:Trifluoperazine and fibroblasts by inhibiting the secretion of TGF-β1andinhibits the ability of fibroblasts to the excessive value, inhibit excessive proliferation offibroblasts and reduces scar tissue levels of TGF-β1and reduces collagen synthesis andaccumulation, and the beginning of the formation of scar formation after both be adjustedto achieve the purpose of inhibiting the formation of excessive scar tissue. For thetreatment of hypertrophic scars caused by the disease only out a direction for the clinicaltreatment of hypertrophic scars using trifluoroacetic provide experimental support. |
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