Effects Of NBD / RADA-16 On Osteogenesis Differentiation Of MC3T3-E1

Objective:Mouse pre osteoblasts MC3T3E1were inoculated and cultured in newfunctional self-assembled hydrogel NBD/RADA16to observe growth condition of MC3T3E1, detect key proteins that affect the differentiation of it,and search the effect ofNBD/RADA16on the ability of proliferation and differentiation of MC3T3E1.Methods:Frozen MC3T3-E1recovered, isolated cultured and passaged; orderedpeptide powder and formed new functional self-assembled hydrogel;selected well-grownsecond generation cell inoculation in hydrogel NBD/RADA-16and3D cells cultured invitro. The control group is the pure RADA16hydrogel. Using MTT assay to detectvalue-added ability;using enzyme standard instrument to detect alkaline phosphatase(alkaline phoaphatase, ALP) activity.21days later, after the induction training for cellalizarin red staining. Using Western Blot appraisal the expression of cells Bonemorphogenetic protein2(BMP2).Results: MC3T3E1cells grew well in NBD/RADA16peptide hydrogel; comparedwith the control group pure RADA16peptide hydrogel, the expression of ALP andmineralized matrix deposition of the experimental group increase, the difference wasstatistically significant (P<0.01), compared with the control group, the Western Blotanalysis showed that the osteogenesis differentiation related protein expression of BMP-2significantly raised.Conclusion: NBD/RADA-16is conducive to the growth and proliferation ofMC3T3-E1,while it can promote osteoblast differentiation.