Expression Of JAK2 Gene In Ph - Negative Myelodysplastic Tumor And Its Clinical Significance

Objective:To investigate the expression of JAK2in patients with Ph-negative myeloproliferative neoplasms(MPN) and its clinical significance.Methods:Conventional karyotype analysis was used for selecting Ph-negative MPN patients, the JAK2mRNA levels in bone marrow samples of MPN patients, secondary erythrocytosis and secondary thrombocythemia(secondary group), and healthy controls were detected by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) technique.Results:The average level of JAK2mRNA in Ph-negative MPN patients, secondary group and healthy controls were0.63±0.35,0.62±0.43and0.60±0.25, respectively. The difference of JAK2mRNA levels between MPN patients and secondary control and healthy control was not significant(P>0.05).Conclusion:Karyotype analysis has important clinical significance for diagnosis and classification in MPN;The JAK2mRNA expression detection alone can not provide a theoretical basis for differential diagnosis for MPN, secondary erythrocytosis, and secondary thrombocythemia.