Genetic Diversity And Phylogeny Of Silicate Bacteria Isolated From Purple Soil

The genetic diversity of 38 silicate bacterium strains isolated from purple soils in Sichuan and Chongqing provinces, together with two reference strains, were analyzed by RAPD, BOX-PCR, 16S rRNA PCR-RFLP, and the phylogeny of the silicate bacteria were determined by 16S rRNA sequences.The results of RAPD analysis, included single primer RAPD and TP-PCR, indicated that all the strains were divided into 5 groups at the similarity of 26%. There were 4 strains in group I, III and V, and 8 strains in group II. Of all the clusters, cluster IV was the biggest one, which contained 18 silicate bacteria from purple soil and 2 reference strains (B. mucilaginosus VKPM 7519~T and AS1.232). The result of TP-PCR analysis was familiar with that of single primer RAPD, and 5 groups were formed at the similarity of 23%. There were 5 strains in group I, 8 strains in group II, 2 strains in group III and 5 strains in group V. In group IV, there were also 18 strains which were grouped with B. mucilaginosus VKPM 7519~T and B. mucilaginosus AS1.232. Which showed that silicate bacteria from purple soil were very diverse. In the combined analysis of the single primer RAPD and TP-PCR, the results were similar to that of single primer RAPD and TP-PCR, and there were 17 strains grouped with B. mucilaginosus VKPM 7519~T and AS1.232 in group I.On the basis of BOX-PCR analysis, all strains were divided into 4 genetic groups at the similarity of 54%, and 32 strains grouped into group II and III, and most strains were relative to the reference strains.In 16S rRNA PCR-RFLP analysis, there were 21 RFLP genotypes existed. At the level of 62% similarity, all strains were divided into three 16S rRNA groups, and there were 24 strains in group I, which was similar to reference stains B. mucilaginosus VKPM 7519~T and AS1.232. There were 10 and 3 strains fall into group II and group III, respectively, and they were distinguished from B. mucilaginosus.Then, 16S rDNA partial sequence of strain K18, and 16S rDNA full sequence of K28 and K38...