The Research To Protecting Effect Of Frozen Semen In Sheep Semen Diluent With Supplementation Of PUFA

On the basis of predecessor research,the study is on the basis of supplementation of glucose 3-3(glucose-citrate- yelk),again supplementation of fish oil enriched PUFA and VE in frozen sheep semen as the new additive, furthermore by testing a series of indexes, researching the protected effect of PUFA to the sheep frozen- thawed semen and the effect of PUFA to sheep frozen- thawed semen fecundation rate.The objective of the research is the effect on sheep frozen ram semen diluent with supplementation of different proportion VE and PUFA ,after frozen-thawed,test usual indexes such as thawed semen motility and intact acrosome ,and Ca2+,Na+,Cl-,LDH enzyme vitality and GOT enzyme vitality etc; at the same time after comparison, ascertain the optimum mixture ration and test thawed semen and fresh semen ultrastructure by transmission electron microscope.The results show that:1. The effect of supplementation of 1% VE and 3% PUFA in sheep thawed semen diluent is less to the sperms harm than the control one after frozen-thawed.every index is all improved after thaw, semen motility and intact acrosome rate are increased respectively by 6% and 10% than the control one(P<0.05),LDH enzyme vitality and GOT enzyme vitality of the semen are reduced respectively by 65% and 15% (P<0.05);2.The semen motility and intact acrosome rate are significantly increased than the control one(P<0.05) with supplementation of PUFA 0.5%,1%,2%,3%,5% after thaw;at the same time,there is a increasing tendency of the semen motility and intact acrosome rate after thaw while PUFA concentration is increased;but which are comparatively lower than the treatment group of supplementation VE ;3. The semen motility(0.345) and intact acrosome rate(0.396)are significantly increased than the other treatment groups with the supplementation of 1% VE in sheep frozen semen diluent and the supplementation of 3% PUFA in the diluent by 2-step method of cryopreservation procedure(P<0.05), LDH enzyme vitality and GOT enzyme vitality of the semen are significantly reduced than the other treatment groups (P<0.05);4. The semen motility(0.328) and intact acrosome rate(0.335)are significantly increased than the control group and the other treatment ones with the supplementation of 3% VE in sheep frozen semen diluent and the supplementation of 3% PUFA in the diluent by 2-step method of cryopreservation procedure(P<0.05), LDH enzyme vitality and GOT...