Analysis On Gliadin And Oil Percentage, Fatty Acid Of Safflower Seeds

Safflower (Carthamus tinctorius L.) is the only cultivated crop in the genus Carthamus L.(Compositae) which is of high value for its oil, remedy and industry utilization, and of great potential for exploitation. Sichuan province is one of the famous safflower cultivated areas for its flower utilization, but not for oil utilization. In this paper, A-PAGE technology was used to analyze the genetic diversity of gliadin of safflower seeds from different countries and the oil and fatty acid percentage of these accessions were also analyzed after immigrated to Sichuan, which provids foundation for the protection and effectively utilization of safflower resource. The main results were as following:1. Gliadin of 53 safflower accessions from 32 countries was analyzed by using acid poiyacrylamide gel electrophoresis technology. Totally, 43 different gliadin band types and 15 gliadin bands with different mobilities were detected. For each accession, 5-12 distinct gliadin bands were detected, with the average of 8.5 per accession, but only one common band was found among all accessions. So gliadin polymorphism within safflower germplasm was high (93.33%). The genetic similarities (GS) values between each two accessions ranged from 0.375 to 1.000, with an average of 0.752. The mean GS values within different continents were 0.759, of which the mean GS value of European accessions were the lowest, while the mean GS values of Asian accessions were the highest. The mean GS values between each two different continents were very close. The mean GS value between African and North American accessions was the highest (0.794), while the lowest was between Asian and European accessions with the mean GS value of 0.745. With the mean GS value 0.752, all accessions were clustered into 6 groups by cluster analysis. The genetic relationship of most accessions based on the gliadin were little associated with its geographical distribution.