Purity Identification And DNA Fingerprinting Database Of Part Main Brassica Napus Hybrids And Their Parents In Guizhou

In this study, morphology method and the SSR marker method were used to analyze 43 brassica napus materials including guiza series hybrid and their parents etc. Differences between the two method were discussed. High multi- state property SSR primer were selected and used in building DNA fingerprinting database of guiza series hybrid and their parents. The main results were as follows:1.Morphology method were usually used to observe characteristic of brassica napus hybrids and their parents in seed identification and purity.but this method is not exact.2.24 brassica napus inbred lines were analyzed by using SSR technics. Twenty-one pairs out of one hundred pairs of SSR primers were selected to gave polymorphic, stable and repeated amplification profiles. Four primers including Nal2C06, Na100B04b, O110A05, Na10D03 were selected to build the DNA fingerprinting database of all 24 inbred lines.3.DNA fingerprint map of 19 brassica napus hybrids were built with 21 pairs SSR primers. These primers also gave polymorphic, stable and repeated amplification profiles. Four primers including Nal2C06, Nal0B04b, O110A05, Na10D03 were selected to build the DNA fingerprinting database of all 19 brassica napus hybrids.4. Test in this research proved that SSR technics can be used in identifying the purity and reality of brassica napus hybrids quickly and precisely.5.Useing primer-in-group method can expand the core primers quickly to identify the purity and reality of brassica napus hybrids.