The Effect Of The Inter-action Of Organic Acid And Honey Material On The Productivity Of Dairy Cow

In conclusion, the experiment was selected four objects of study, including citric acid, malic acid iso-butyric acid and honey material. And then the experiment select 16 sound dairy cow, according to age, fetal frequency, calving period, BW and feeding. The experiment was adopted orthogonal experiment design with four factors and two levels. Studying the effects of feed additive on balance feed nutrient correcting palatability, improving nutrition and digestive, maintaining and enhancing ruminant microorganism fermentation.The experiment was included the following test item and the following method. First, period weight: during the beginning and the end of this experiment, before dairy cows are feed on the morning trial dairy cows are weighted respectively. Second, produce body condition scoring in every period of dairy cow. Third, record time of breeding. Fourth, recording DIM : recording the amount of feeding every cow every day. Fifth, record milk yield: recording the quantity of milk twice every week and accumulate morning milk yield, noon milk yield and event milk yield and then statistics milk yield all day along. Set out milk fat percentage, recording milk yield, at t-he same time, take a sample of 10ml milk sample respectively and setting-out milk fat percentage. Seventh, set out milk protein percentage; recording milk yield, at the same time, picking up 10ml milk sample respectively and setting-out milk protein percentage. Eighth, taking a sample of 500g trial diet during the 10th day, the 20th day and the 30th day respectively, and then crush trial diet with grinder, at the same time, take a sample of 500g cow dung respectively and mix them with 10 drops of H₂SO₄, and then preserve them at -20 centigrade. Ninth, collecting the blood sample and preparation: collecting the blood samples in jugular vein, and then centrifuging them with a centrifuge at 3000r/min, at last, setting-out glucose, albumin, total protein and urea nitrogen.Feeding experiment result: First, feeding of dry matter: the constitute of citric acid, malic acid and honey material have an important effect on flavoring, and enhance feeding of dry matter. Second, variety of weight: trial dairy cows have a variety of weight. Citric acid and malic acid have a slight influence on that. Body condition scoring about dairy cow has little variety. Third, the variety of milk yield, milk fat percentage and milk protein percentage: milk yield haveextremely significant difference (p<0.01). A constitute of malic acid and honey material have the most remarkable influence on milk yield. So does citric acid (p<0.01). But synthesize all of the data, joint function of iso-butyric acid and honey material has significant difference (p<0.05).Digestive experiment results: First, feed additive have unremarkable influence on DE. A constitute of the malic acid and iso-butyric have slightly influence at low level. Second, feed additive have unremarkable influence on digestibility of fat. A constitute of malic acid and honey material have slightly influence at low level. So does iso-butyric acid. Third, feed additive have significant difference on ADF (p<0.05). A constitute of malic acid and iso-butyric acid have significant difference on ADF (p<0.05). Third, feed additive have significant difference on ADF (p<0.05). A constitute of malic acid and iso-butyric acid have extremely significant difference on NDF (p<0.01). And, joint function of malic acid and iso-butyric acid has extremely significant difference on NDF at high level, too (p<0.01). Fifth, feed additive have no significant difference on crude protein. Malic acid has slight influence. Sixth, set out blood items results. Feed additive have little influence on blood items. When synthesized the data, malic acid, iso-butyric acid have slight influence on total protein; iso-butyric acid has an slightly influence on albumin; citric acid has an influence on citric acid; citric acid and iso-butyric acid have an influence on urea nitrogen. But they have no significant difference. Conclusion:First, the constitute of citric acid, malic acid and honey material are made into flavoring so that it can improve feeding of rough forage. The ultimate ratio is 20g/d of citric acid, 40g/d of malic acid and 9g/d of honey material.Second, the inter-action of malic acid and iso-butyric acid has significant difference on apparent digestibility of ADF, NDF and fat. . The ultimate ratio is 20g/d of malic acid and lOg/d of iso-butyric acidl.Third, citric acid can improve milk yield . The ultimate ratio is 20g/d of citric acidFourth, the experiment has no bad effect on blood index.