Research On Biological Activity Of Phellinus Igniarius's Giant Protoplast And Its Function

This experiment includes two parts based on Phellinus igniarisu.In the first part, biological characteristic of gigant protoplast separated from Phellinus igniarius was studied. We observed that isolation, regeneration of giant protoplast and its ultrastructural process.The results showed that Phellinus igniarius could yield large numbers of giant protoplast dealed with 3% lywallzyme for seven days within 37 ℃. Its diameter ranged from 13 to 20μm.The mecylia formed when giant protoplast were inoculated on regeneration medium and cultured for eleven days at 25°C .Compared with general protoplast,great changes happened on giant protoplast by TEM.At first,cell structure of giant protoplast were affected by long time and the gap came into being between cell membrane;secondly,vacuole in the giant protoplast became obviously larger than before;at last,numbers and vacuum of mt also became more and larger.In addition, we didn' t discover the phenomenon of nucleolus abnormal.ln the second part, water-solubilable polysaccharide was extracted by boiling water, isolated and purified by all varieties of bio-chemical techonologies.Two main apex were separated by SephadexG-200 gel coloum.Gel electrophoresis experiments showed that each polysaccharide apex was very pure and their molecular weight were forty-seven hundred thousand and one hundred thousand respectively.Polysaccharide had very strong biological activities after it was purified.It could improve the expression of cytokines like IL-2 in the body of rat,could increase antioxidant enzyme activity and enhance the ability of body to scanvage free radicals and reduce the damage caused by free radicals,meanwhile it could prompt microcircles of liver and restore liver cells,decrease the release of ALT and ALT,so the polysaccharide had protective effect on chemical liver injury caused by CCl4.During the study,we observed that polysaccharide could inhibit the appearance of fatty liver.ln vitro antitumor experiment proved that polysaccharide possessed direct cell toxicity because of its inhibition effect on Hela tumor cell.