Studies On Elimination And Detection Of Viruses In Cherry

The　methods　of　obtaining　sweet　cherry　free　from　viruses　through　stem　apex　culture,thermothetapy　combined　with　stem　apex　culture　and　treatment　with　Ribavirin　were　studied,12　cultivor　sweet　cherries　as　trial　materials　in　this　work.The　results　were　showed:(1)survival　rate　was　internal　relative　with　genotype;(2)survival　rate　was　positive　correlation　with　length　of　stem　apex;(3)survival　rate　was　extremely　low　after　thermotherapy　;(4)survival　rate　was　affected　by　concentration　and　time　of　treatment　with　Ribavirin　and　the　effect　of　concentration　was　more　visible　than　that　of　time.The　virus　detection　results　for　PNRSV　and　PDV　by　ELISA　and　RNA　slide　hybridization　were　indicated:cultivars　R-C,A31,W-1,W-2,1-1,KN,S,Y,D　were　uninfected　by　PNRSV　and　PDV,cultivars　A30,07,01　were　infected　by　PNRSV　only　and　parts　of　these　three　cultivars　after　virus-elimination　were　virus-free.The　results　were　showed:(1)obtaining　sweet　cherry　free　from　PNRSV　through　stem　apex　culture　was　viable,virus-free　rate　was　negative　correlative　with　length　of　stem　apex;(2)virus-free　rate　was　increased　by　multi-stem　apex　culture　;(3)best　of　　these　three　methods　was　　thermothetapy　combined　with　stem　apex　culture,and　maximum　virus-free　rate　was100%;(4)Ribavirin　was　ineffective　to　eliminate　PNRSV　in　sweet　cherry　in　this　study.